| 台湾乳白蚁纤维素降解菌的分离和鉴定 |
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| 引用本文： | 陈志超,易弋,赵东玲,黄翠姬,伍时华.台湾乳白蚁纤维素降解菌的分离和鉴定[J].中国酿造,2019,38(1):54. |
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| 作者姓名： | 陈志超 易弋 赵东玲 黄翠姬 伍时华 |
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| 作者单位： | （1.广西科技大学 生物与化学工程学院，广西 柳州 545006；2.广西科技大学 广西糖资源绿色加工重点实验室，广西 柳州 545006；3.广西科技大学 广西高校糖资源加工重点实验室，广西 柳州 545006） |
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| 基金项目： | 国家自然科学基金（31660250，31560728） |
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| 摘 要： | 该研究从广西柳州本地的台湾乳白蚁（Coptotermes formosanus）体内筛选一株纤维素降解菌，通过形态观察、生理生化试验以及分子生物学对其进行鉴定，并采用滤纸片法及3,5-二硝基水杨酸（DNS）法测定该菌株的纤维素酶活力。结果表明，筛选获得一株可在以羧甲基纤维素钠为唯一碳源的培养基上生长的菌株，命名为BW，并鉴定其为约氏不动杆菌（Acinetobacter johnsonii）；菌株BW的纤维素酶活力最高，为（93.73±0.22）U/mL。
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| 关 键 词： | 台湾乳白蚁 纤维素降解菌 分离 鉴定 纤维素酶 |
Isolation and identification of cellulose-degrading bacteria from Coptotermes formosanu |
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| CHEN Zhichao,YI Yi,ZHAO Dongling,HUANG Cuiji,WU Shihua.Isolation and identification of cellulose-degrading bacteria from Coptotermes formosanu[J].China Brewing,2019,38(1):54. |
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| Authors: | CHEN Zhichao YI Yi ZHAO Dongling HUANG Cuiji WU Shihua |
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| Affiliation: | (1.College of Biological and Chemical Engineering, Guangxi University of Science and Technology, Liuzhou 545006, China; 2.Guangxi Key Laboratory of Green Processing of Sugar Resources, Guangxi University of Science and Technology, Liuzhou 545006, China;3.Key Laboratory for Processing of Sugar Resources of Guangxi Higher Education Institutes, Guangxi University of Science and Technology, Liuzhou 545006, China) |
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| Abstract: | A cellulose-degrading bacterium was screened from Coptotermes formosanus in Liuzhou, Guangxi, and identified by morphological observation, physiological and biochemical tests and molecular biology. The cellulase activity of the strain was measured by filter paper method and 3,5-dinitrosalicylic acid (DNS) method. The results showed that the strain which could grew on the medium using carboxymethylcellulose sodium as the only carbon source was obtained, named as BW and identified as Acinetobacter johnsonii. The cellulase activity of strain BW was the highest and reached (93.73±0.22) U/ml. |
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| Keywords: | Coptotermes formosanus cellulose-degrading bacteria isolation identification cellulase |
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