| 山西老陈醋高产糖化酶霉菌的筛选、鉴定及产酶条件优化 |
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| 引用本文： | 王佳丽,朗繁繁,陈旭峰,王如福,许女. 山西老陈醋高产糖化酶霉菌的筛选、鉴定及产酶条件优化[J]. 中国酿造, 2019, 0(4): 100-105 |
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| 作者姓名： | 王佳丽 朗繁繁 陈旭峰 王如福 许女 |
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| 作者单位： | 山西农业大学食品科学与工程学院;山西紫林醋业股份有限公司;山西农业大学实验教学中心 |
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| 基金项目： | 山西省应用基础研究项目(201701D221189);山西省科技重大专项项目(201703D211001-06-05) |
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| 摘 要： | 从山西老陈醋大曲中共分离出75株霉菌,对高产糖化酶霉菌进行筛选、鉴定及产酶条件优化。结果表明,筛选出1株优良黑曲霉(Aspergillus niger)186,在麸皮固态培养基中其糖化酶、蛋白酶和纤维素酶酶活分别为3 963.79 U/g、368.80 U/g和4 476.60 U/g。糖化酶基因聚合酶链反应(PCR)测序结果显示,该酶脱氧核糖核酸(DNA)序列编码区长1 449 bp,共编码482个氨基酸,预测酶的分子质量为51.75 kDa,等电点为3.99。最佳产酶条件为:麸皮4%,硫酸铵0.5%,初始pH值为7.0,接种量8%,装液量100 m L/250 m L,转速145 r/min,培养7 d。在此优化条件下,黑曲霉186糖化酶活力可达892.98 U/m L,是优化前的1.77倍。
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| 关 键 词： | 山西老陈醋 霉菌 筛选 鉴定 糖化酶 条件优化 |
Screening and identification of mold with high-yield saccharifying enzyme from Shanxi mature vinegar and optimization of enzyme production conditions |
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| WANG Jiali,LANG Fanfan,CHEN Xufeng,WANG Rufu,XU Nv. Screening and identification of mold with high-yield saccharifying enzyme from Shanxi mature vinegar and optimization of enzyme production conditions[J]. China Brewing, 2019, 0(4): 100-105 |
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| Authors: | WANG Jiali LANG Fanfan CHEN Xufeng WANG Rufu XU Nv |
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| Affiliation: | (College of Food Science and Engineering,Shanxi Agricultural University,Jinzhong 030801,China;Shanxi Zilin Vinegar Co.,Ltd.,Taiyuan 030400,China;Experimental Teaching Center,Shanxi Agricultural University,Jinzhong 030801,China) |
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| Abstract: | WANG Jiali;LANG Fanfan;CHEN Xufeng;WANG Rufu;XU Nv(College of Food Science and Engineering,Shanxi Agricultural University,Jinzhong 030801,China;Shanxi Zilin Vinegar Co.,Ltd.,Taiyuan 030400,China;Experimental Teaching Center,Shanxi Agricultural University,Jinzhong 030801,China) |
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| Keywords: | Shanxi mature vinegar mold screening identification saccharifying enzyme conditions optimization |
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