| 小麦麸皮水不溶性阿拉伯木聚糖提取及其酶解产物分析 |
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| 引用本文： | 黎芳,刘佳,王冉冉,刘必慧,李冲,易若琨,赵欣. 小麦麸皮水不溶性阿拉伯木聚糖提取及其酶解产物分析[J]. 中国酿造, 2019, 38(2): 122-126. DOI: 10.11882/j.issn.0254-5071.2019.02.023 |
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| 作者姓名： | 黎芳 刘佳 王冉冉 刘必慧 李冲 易若琨 赵欣 |
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| 作者单位： | （1.重庆第二师范学院 生物与化学工程学院 重庆市功能性食品协同创新中心 重庆市功能性食品工程技术研究中心 重庆市功能性食品研发工程实验室，重庆 400067；2.重庆第二师范学院 生物与化学工程学院，重庆 400067） |
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| 基金项目： | 重庆高校创新团队建设计划资助项目(CXTDX201601040) |
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| 摘 要： | 以小麦麸皮为原料，采用碱提法对小麦麸皮中的水不溶性阿拉伯木聚糖进行提取。以水不溶性阿拉伯木聚糖得率为响应值，采用单因素试验和响应面分析法对其提取工艺进行优化，并利用不同木聚糖酶对其进行酶解，采用薄层色谱（TLC）法对酶解产物进行分析。结果表明，水不溶性阿拉伯木聚糖的最佳提取工艺为料液比1∶193（g∶mL）、提取温度61 ℃、提取时间5 h。在此最优提取工艺条件下，水不溶性阿拉伯木聚糖的得率为51.61%，较优化前提高20.71%。用不同种类木聚糖酶对提取的水不溶性阿拉伯木聚糖进行酶解，TLC分析结果表明，链霉菌10904来源的木聚糖酶A对水不溶性阿拉伯木聚糖有较好的底物特异性，酶解产物丰富且以木二糖为主，为阿拉伯低聚木糖的制备提供理论依据。
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| 关 键 词： | 小麦麸皮 水不溶性阿拉伯木聚糖 碱提法 响应面分析 酶解产物 |
Extraction of water insoluble arabinoxylan from wheat bran and analysis of its enzymatic hydrolyzed products |
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| LI Fang,LIU Jia,WANG Ranran,LIU Bihui,LI Chong,YI Ruokun,ZHAO Xin. Extraction of water insoluble arabinoxylan from wheat bran and analysis of its enzymatic hydrolyzed products[J]. China Brewing, 2019, 38(2): 122-126. DOI: 10.11882/j.issn.0254-5071.2019.02.023 |
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| Authors: | LI Fang LIU Jia WANG Ranran LIU Bihui LI Chong YI Ruokun ZHAO Xin |
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| Affiliation: | (1.Chongqing Engineering Laboratory for Functional Food, Chongqing Engineering Research Center of Functional Food, Chongqing Collaborative Innovation Center for Functional Food, School of Biological & Chemical Engineering, Chongqing University of Education, Chongqing 400067, China; 2.School of Biological & Chemical Engineering, Chongqing University of Education, Chongqing 400067, China) |
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| Abstract: | LI Fang;LIU Jia;WANG Ranran;LIU Bihui;LI Chong;YI Ruokun;ZHAO Xin(Chongqing Engineering Laboratory for Functional Food, Chongqing Engineering Research Center of Functional Food, Chongqing Collaborative Innovation Center for Functional Food, School of Biological & Chemical Engineering, Chongqing University of Education, Chongqing 400067, China;School of Biological & Chemical Engineering, Chongqing University of Education, Chongqing 400067, China) |
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| Keywords: | wheat bran water insoluble arabinoxylan alkali extraction method response surface analysis enzymatic hydrolyzed products |
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