| 高产脂肪酶菌株的筛选鉴定及产酶条件优化 |
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| 引用本文： | 刘延波,邢星月,赵志军,刘宁,潘春梅,孙西玉. 高产脂肪酶菌株的筛选鉴定及产酶条件优化[J]. 中国酿造, 2019, 38(7): 54. DOI: 10.11882/j.issn.0254-5071.2019.07.011 |
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| 作者姓名： | 刘延波 邢星月 赵志军 刘宁 潘春梅 孙西玉 |
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| 作者单位： | （1.河南牧业经济学院 食品与生物工程学院（酒业学院），河南 郑州 450046；2.河南牧业经济学院 河南省白酒风格工程技术研究中心，河南 郑州 450046；3.河南张弓老酒酒业有限公司，河南 宁陵 476733） |
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| 基金项目： | 河南牧业经济学院博士科研启动资金项目；河南牧业经济学院青年科研创新基金项目[XKYCXJJ2017015]；河南省高等学校重点科研项目[17A180005]；河南省重大科技专项[181100211400]；河南牧业经济学院重点学科项目 |
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| 摘 要： | 采用罗丹明B平板法和脂肪酶活力测定从张弓老酒大曲中分离筛选出一株高产脂肪酶菌株E-11，经形态学、生理生化试验和16S rDNA序列分析，鉴定该菌株为枯草芽孢杆菌（Bacillus subtilis）。通过单因素和响应面试验设计对该菌株的产酶条件进行优化，结果表明，最佳产酶条件为葡萄糖3%、蛋白胨4%、初始pH值为8.0。优化后的脂肪酶活力达15.09 U/mL，是优化前产酶能力的1.2倍。
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| 关 键 词： | 大曲 脂肪酶 分离鉴定 响应面试验 发酵条件优化 |
Screening and identification of high yield lipase strains and optimization of enzyme production conditions |
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| LIU Yanbo,XING Xingyue,ZHAO Zhijun,LIU Ning,PAN Chunmei,SUN Xiyu. Screening and identification of high yield lipase strains and optimization of enzyme production conditions[J]. China Brewing, 2019, 38(7): 54. DOI: 10.11882/j.issn.0254-5071.2019.07.011 |
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| Authors: | LIU Yanbo XING Xingyue ZHAO Zhijun LIU Ning PAN Chunmei SUN Xiyu |
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| Affiliation: | (1.College of Food and Biological Engineering (Liquor College), Henan University of Animal Husbandry and Economy, Zhengzhou 450046, China; 2.Henan Liquor Style Engineering Technology Research Center, Henan University of Animal Husbandry and Economy, Zhengzhou 450046, China; 3.ZhangGongLaoJiu Wine Co., Ltd., Ningling 476733, China) |
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| Abstract: | A high yield lipase strain E-11 was isolated and screened from Daqu of Zhanggonglaojiu by rhodamine B plate method and lipase activity determination. After morphological, physiological and biochemical tests and 16S rDNA sequences analysis, the strain was identified as Bacillus subtilis. The optimum conditions of enzyme production were optimized by single factor tests and response surface methodology and were determined as follows: glucose 3%, peptone 4%, and initial pH 8.0. Under the optimal conditions, the lipase activity reached 15.09 U/ml, which was 1.2 times that of before optimization. |
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| Keywords: | Daqu lipase isolation and identification response surface methodology fermentation conditions optimization |
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