一种新型喹唑啉酮衍生物A与牛血清白蛋白作用的研究

应用荧光光谱和紫外可见吸收光谱研究了A与牛血清白蛋白间的结合作用,确定了A对牛血清白蛋白的荧光猝灭过程的猝灭机理。测定了不同温度下该结合反应的结合常数,结合位点数,热力学参数。依据能量转移理论确定了A和蛋白间的结合距离。采用同步荧光技术考察了A对牛血清白蛋白构象的影响,并讨论了A与蛋白的结合模式。

Studies on the Interaction Between A and Bovine Serum Albumin

The binding between A and bovine serum albumin(BSA)was investigated by fluorescence spectrum and UV spectrum.It is proved that the fluorescence quenching of BSA by A is a result of A-BSA complex.The binding constant,the number of binding sites n and thermodynamic parameters were measured at different temperatures by fluorescence quenching method.The binding distance r between A and BSA was also obtained according to Forster theory of non-radiation energy transfer.The effect of A on the conformation of BSA h...