Procedure for red blood cell labelling with 99mTc-HMPAO. Methodology and quality control |
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Authors: | P Bassa JR García Garzón C Piera A Pavía M Minoves M Moragas J Pavía F Lome?a J Setoain |
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Affiliation: | Nuclear Medicine Service, Hospital Clinic I Provincial de Barcelona, Spain. |
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Abstract: | We present here results on the labelling of red blood cells with 99mTc-HMPAO as an alternative method to the usual in vitro technique. Anticoagulant agents, the labelling medium with plasma, and the lapse of time between 99mTc-HMPAO preparation and labelling are the main factors which affect the efficiency of the procedure. A 93.9 +/- 2.3% labelling yield was obtained with freshly prepared 99mTc-HMPAO. In vitro (tracer elution of 4.3 +/- 1.2% at 60 mins) and in vivo (percentage of plasma activity at 60 mins, 7.8 +/- 2.8%) stability of the label, as well as image quality, qualify 99mTc-HMPAO labelled red blood cells as a suitable agent for clinical use. |
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