烟草感染赤星病后有关酶动态的研究

本文研究１１个烟草品种感染赤星病后过氧化物酶（ＰＯＤ）同工酶、多酚氧化酶（ＰＰＯ）、硝酸还原酶（ＮＲ）和抗坏血酸氧化酶（ＡＡＯ）活性的变化规律。结果表明：抗赤星病强的品种，ＰＰＯ在接种３６ｈ内活性升高，以后下降；ＰＯＤ同工酶活性变幅大而快，接种初期活性稍有下降，然后增强，其中以阴极端２、４同工酶带增加较快，接种１８０ｈ后就由弱酶带变为强带；ＮＲ活性变化不大；ＡＡＯ活性提高。抗赤星病弱的品种，ＰＰＯ活性低于对照；ＰＯＤ同工酶变化不大，呈下降趋势；ＮＲ活性变幅大；抗坏血酸氧化酶在接种初期活性下降，８４ｈ后回升。

Study on the Dynamics of Some Related Enzymes of Tobacco infected with Brown Spot

This article is a study about the activity changing patterns of POD, PPO, NR and AAO in 11 tobacco varieties infected with brown spot disease. Results showed that varieties with strong resistance to this disease with their PPO activity went up after 36 hours of innoculation and went down afterwards. The POD activity changing was fast and the scope was wider at early stage of innoculation, the activity was down a little and then increased, the more increased belonged to 2, 4 isozymogram band on the cathode.After innoculated 180 hours it was changed from weak to strong band. Activity of NR changed little. Activity of AAO increased. Varieties with weak resistance to brown spot were lower with their PPO activities than that of CK. POD had little change and it tended to decrease. Activity of NR declined at early stage of innoculation.