| RP-HPLC用于芦荟多糖的单糖组成研究 |
| |
| 引用本文： | 林雪,贾敬芬,黄琳娟,王仲孚. RP-HPLC用于芦荟多糖的单糖组成研究[J]. 食品科学, 2006, 27(4): 192-195 |
| |
| 作者姓名： | 林雪 贾敬芬 黄琳娟 王仲孚 |
| |
| 作者单位： | 西北大学生命科学学院西部资源生物与现代生物技术省部共建教育部重点实验室 |
| |
| 摘 要： | 采用水提醇沉法提取芦荟多糖,用三氟乙酸(TFA)水解,通过1-苯基-3-甲基-5-吡唑啉酮(PMP)衍生水解后的单糖,衍生物用反相高效液相色谱(RP-HPLC)分析,紫外检测,研究了三种常见芦荟多糖的单糖组成及摩尔比。结果表明:利用RP-HPLC研究芦荟多糖的单糖组成简便易行,并且芦荟多糖因品种不同存在单糖组成差异。从而建立了研究芦荟多糖的新方法。
|
| 关 键 词： | 芦荟多糖 衍生化 1-苯基-3-甲基-5-吡唑啉酮 反相高效液相色谱 |
| 文章编号： | 1002-6630（2006）04-0192-04 |
| 收稿时间： | 2005-06-27 |
| 修稿时间： | 2005-06-27 |
Study on the Use of RP-HPLC in Analysis of Aloe Polysaccharide Composition |
| |
| LIN Xue,JIA Jing-fen,HUANG Lin-juan,WANG Zhong-fu. Study on the Use of RP-HPLC in Analysis of Aloe Polysaccharide Composition[J]. Food Science, 2006, 27(4): 192-195 |
| |
| Authors: | LIN Xue JIA Jing-fen HUANG Lin-juan WANG Zhong-fu |
| |
| Affiliation: | Key Laboratory of Ministry of Education for Western China Resource Biology and Biotechnology, College of Life Science, Northwest University, Xi’an 710069, China |
| |
| Abstract: | In this article, Aloe polysaccharide was extracted by water and precipitated by ethanol. The polysaccharide was hydrolyzed by TFA. A method for the derivatization of polysaccharide hydrolysate with 1-phenyl-3-methyl- 5-pyrazolone (PMP) was used to study the composition and molar ratio of three different species of Aloe by RP-HPLC. Results showed that the method of using RP-HPLC on studing Aloe polysaccharide composition is simple and convienient, and the Aloe polysaccharidescontent and sugar composition show differences among different species. Thus,a new method to study Aloe polysaccharide composition is established. |
| |
| Keywords: | Aloe polysaccharide derivatization 1-phenyl-3-methyl-5-pyrazolone RP-HPLC |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《食品科学》浏览原始摘要信息 |
|
点击此处可从《食品科学》下载全文 |
