| 磁性聚丙烯酰胺-丙烯酸共聚纳米粒子固定脂肪酶的研究 |
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| 引用本文： | 王燕佳,蒋惠亮,方银军,任国晓. 磁性聚丙烯酰胺-丙烯酸共聚纳米粒子固定脂肪酶的研究[J]. 应用化工, 2008, 37(5): 491-494 |
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| 作者姓名： | 王燕佳 蒋惠亮 方银军 任国晓 |
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| 作者单位： | 1. 江南大学化学与材料工程学院,江苏无锡,214122
2. 浙江赞宇科技有限公司,浙江杭州,310009 |
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| 摘 要： | 用沉淀聚合制备了P(Am-co-Aa)-Gd(Ⅲ)磁性高分子纳米微球,在此基础上通过共价键合固定脂肪酶。结果表明:固定脂肪酶后的磁性纳米微球具有优异的磁分离能力;钆离子对固定化酶有明显的激活作用,当钆离子质量分数为0.8%时,偶联率和活力回收率分别提高57%和60%;脂肪酶被固定化后其pH稳定性,操作稳定性均比自由酶明显提高。
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| 关 键 词： | 纳米粒子 Gd(Ⅲ) 脂肪酶 固定 磁性 |
| 文章编号： | 1671-3206(2008)05-0491-04 |
| 修稿时间： | 2008-03-20 |
Study on immobilization of lipase with P (Am-co-Aa) magnetic nanoparticle |
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| WANG Yan-jia,JIANG Hui-liang,FANG Yin-jun,REN Guo-xiao. Study on immobilization of lipase with P (Am-co-Aa) magnetic nanoparticle[J]. Applied chemical industry, 2008, 37(5): 491-494 |
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| Authors: | WANG Yan-jia JIANG Hui-liang FANG Yin-jun REN Guo-xiao |
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| Abstract: | Based on the preparation of P(Am-co-Aa)-Gd(Ⅲ) magnetic nanoparticles by precipitation polymerization,lipase was immobilized on this nanoparticles with covalent linkage.It was found that immobilized lipase magnetic nanoparticles possessed excellent ability of magnetic separation.Gd(Ⅲ) could activate the immobilized lipase,when the weight ratio of Gd(Ⅲ) is 0.8%,the coupled yield and activity of the immobilized lipase was increased by 57% and 60%,respectively.As compared with free lipase,the pH and operation stabilities of immobilized lipase were increased appreciably. |
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| Keywords: | nanoparticles Gd(Ⅲ) lipase immobilization magnetic |
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