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产纤溶酶米曲霉的筛选及诱变育种
引用本文:高占争,赵允麟.产纤溶酶米曲霉的筛选及诱变育种[J].食品与生物技术学报,2006,25(4):93-96.
作者姓名:高占争  赵允麟
作者单位:江南大学,教育部工业微生物重点实验室,江苏,无锡,214036
摘    要:利用自己设计的筛选方法,从不同的酿造曲样中筛选出1株产纤溶酶酶活较高的米曲霉菌株SA-6,其产纤溶酶酶活为25U/mL。SA-6经过紫外线、^60Co和亚硝基胍诱变,筛选得到突变株NA-25,其产酶酶活为63U/mL,是出发菌株SA-6的2.52倍。突变株NA-25的发酵性状改变,其产纤溶酶的高峰比出发菌株迟了约10h。

关 键 词:纤溶酶  米曲霉  筛选  诱变
文章编号:1673-1689(2006)04-0093-04
修稿时间:2005年6月22日

Screening and Mutagenesis of the Fibrinolytic Enzyme Producing Strain Asperillus oryzae
GAO Zhan-zheng,ZHAO Yun-lin.Screening and Mutagenesis of the Fibrinolytic Enzyme Producing Strain Asperillus oryzae[J].Journal of Food Science and Biotechnology,2006,25(4):93-96.
Authors:GAO Zhan-zheng  ZHAO Yun-lin
Abstract:A Asperillus Oryzae strain,SA-6 was isolated with different screening methods and the fibrinolytic enzyme activity was 25 U/mL.A mutant was then isolated after SA-6 was treated with UV,~(60)Co and NTG and the relevant firbrinolytic enzyme activity was enhanced to 63 U/mL which was 1.52 times higher than that of SA-6.Comparing with the starting strain,the appearance time of the maximum fibrinolytic enzyme production of the mutant was delayed 10 hours.
Keywords:fibrinolytic enzyme  Asperillus Oryzae  screening  mutagenesis
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