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Cost-Effective High-Throughput Fully Automated Construction of a Multiplex Library of Mutagenized Open Reading Frames for an Insecticidal Peptide Using a Plasmid-Based Functional Proteomic Robotic Workcell with Improved Vacuum System |
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| Authors: | Stephen R Hughes Patrick F Dowd Ronald E Hector Steven B Riedmuller Scott Bartolett Jeffrey A Mertens Nasib Qureshi Siqing Liu Kenneth M Bischoff Xin-Liang Li John S Jackson Jr David Sterner Tadas Panavas Joseph O Rich Philip J Farrelly Tauseef Butt Michael A Cotta |
| Affiliation: | 1United States Department of Agriculture (USDA), Agricultural Research Service (ARS), National Center for Agricultural Utilization Research (NCAUR), Peoria, IL;2Hudson Control Group, Inc., Springfield, NJ;3LifeSensors, Inc., Malvern, PA |
| Abstract: | |
| Keywords: | cost-effective fully automated high-throughput molecular biology large-scale optimized open reading frame cloning and bacterial transformation automated amino acid scanning mutagenesis (AASM) library construction automated vacuum block |
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