Fabrication of Calix[]arene Derivative Monolayers to Control Orientation of Antibody Immobilization |
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Authors: | Hongxia Chen Feng Liu Fangjie Qi Kwangnak Koh Keming Wang |
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Affiliation: | 1.Laboratory of Biosensing Technology, School of Life Sciences, Shanghai University, Shanghai 200444, China; E-Mails: (H.C.); (F.L.); (F.Q.);2.Shanghai Key Laboratory of Bio-Energy Crops, School of Life Sciences, Shanghai University, Shanghai 200444, China;3.College of Nanoscience and Nanotechnology, Pusan National University, Pusan 609-735, Korea; E-Mail: ;4.Department of Oncology, the Second Affiliated Hospital of Nanjing Medical University, Nanjing 210011, China |
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Abstract: | Three calix4]arene (Cal-4) derivatives which separately contain ethylester (1), carboxylic acid (2), and crownether (3) at the lower rim with a common reactive thiol at the upper rim were synthesized and constructed to self-assembled monolayers (SAMs) on Au films. After spectroscopic characterization of the monolayers, surface coverage and orientation of antibody immobilized on the Cal-4 derivative SAMs were studied by surface plasmon resonance (SPR) technique. Experimental results revealed that the antibody could be immobilized on the Cal-4 derivatives spontaneously. The orientation of absorbed antibody on the Cal-4 derivative SAMs is related to the SAM’s dipole moment. The possible orientations of the antibody immobilized on the Cal-4 derivative 1 SAM are lying-on or side-on, while on the Cal-4 derivative 2 and Cal-4 derivative 3 head-on and end-on respectively. These experimental results demonstrate the surface dipole moment of Cal-4 derivative appears to be an important factor to antibody orientation. Cal-4 derivatives are useful in developing site direct protein chips. |
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Keywords: | calixarene surface plasmon resonance (SPR) Immunoglobulin G (IgG) orientation dipole moment |
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