| 荧光假单胞菌RB5产嗜铁素的发酵条件 |
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| 引用本文： | 伊艳杰,周广舟,时玉,李瑞芳,陈晓娣.荧光假单胞菌RB5产嗜铁素的发酵条件[J].河南工业大学学报(自然科学版),2011,32(6):32-35,39. |
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| 作者姓名： | 伊艳杰 周广舟 时玉 李瑞芳 陈晓娣 |
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| 作者单位： | 河南工业大学生物工程学院,河南郑州,450001 |
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| 基金项目： | 河南省教育厅自然科学基金(2010A210003); 河南工业大学高层次人才科研基金(2010BS016) |
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| 摘 要： | 荧光假单胞菌RB5是从土壤中分离得到的一株对禾谷丝核菌有拮抗作用的细菌.通过CAS法检测分析,发现RB5菌株能产生嗜铁素.为明确荧光假单胞菌RB5产嗜铁素的发酵条件,采用摇瓶培养发酵,利用特异光谱吸收法,研究了不同培养条件对RB5菌株嗜铁素产量的影响.最终得出RB5产嗜铁素的最佳发酵条件:起始pH 7.0,接种量2％,瓶装量90 mL/250 mL,发酵时间48 h,Fe3+质量浓度为0.8 mg/L.抑菌试验表明,优化发酵条件后的RB5发酵液(OD400值2.35)有很显著的抑菌效果,抑制率达65.28％.
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| 关 键 词： | 荧光假单胞菌 嗜铁素 发酵条件 抑菌效果 |
FERMENTATION CONDITIONS OF SIDEROPHORE PRODUCED BY PSEUDOMONAS FLUORESCENS RB5 |
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| YI Yan-jie
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ZHOU Guang-zhou
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SHI Yu
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LI Rui-fang
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CHEN Xiao-di.FERMENTATION CONDITIONS OF SIDEROPHORE PRODUCED BY PSEUDOMONAS FLUORESCENS RB5[J].Journal of Henan University of Technology Natural Science Edition,2011,32(6):32-35,39. |
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| Authors: | YI Yan-jie ZHOU Guang-zhou SHI Yu LI Rui-fang CHEN Xiao-di |
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| Affiliation: | YI Yan-jie,ZHOU Guang-zhou,SHI Yu,LI Rui-fang,CHEN Xiao-di(School of Bioengineering,Henan University of Technology,Zhengzhou 450001,China) |
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| Abstract: | Pseudomonas fluorescens RB5 antagonistic to Rhizoctonia cerealis was isolated from the soil.Through CAS detection,we found that strain RB5 could secrete siderophores.In order to study the optimum fermentation conditions of siderophore produced by Pseudomonas fluorescens RB5,we studied the influence of different culture conditions on the yield of siderophore from strain RB5 by using shaking flask fermentation and 400-nm specific spectral absorption assay.The results showed that the optimum culture conditions... |
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| Keywords: | Pseudomonas fluorescens siderophore fermentation conditions inhibition effect |
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