| 核磁共振波谱法在多糖结构分析中的应用 |
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| 引用本文： | 刘玉红,王凤山.核磁共振波谱法在多糖结构分析中的应用[J].食品与药品,2007,9(8A):39-43. |
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| 作者姓名： | 刘玉红 王凤山 |
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| 作者单位： | [1]山东大学药学院生化与生物技术药物研究所,山东济南250012 [2]山东中医药大学药学院,山东济南250014 |
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| 摘 要： | 多糖由于结构复杂使解析结构非常繁琐和困难,使对各种多糖的深入研究受限。核磁共振波谱法（NMR）是解析物质结构最有效的手段,近年该技术的发展也很迅速。本文综述了一维核磁共振（1D-NMR）和二维核磁共振波谱法（2D-NMR,包括COSY,TOCSY,ROESY,NOESY,HMQC,HSQC与HMBC）在多糖结构分析中的应用。这些技术可以提供如多糖的单糖组成、单糖残基间的顺序、单糖残基在糖苷键中的位置、环状结构的类型和糖苷键的构型等许多信息,成为分析多糖结构不可缺少的工具。
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| 关 键 词： | 核磁共振波谱法 多糖 结构分析 |
| 文章编号： | 1672-979X（2007）08-0039-05 |
| 修稿时间： | 2007-04-10 |
Applications of Nuclear Magnetic Resonance Spectroscopy in Structural Analysis of Polysaccharides |
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| LIU Yu-hong, WANG Feng-shan.Applications of Nuclear Magnetic Resonance Spectroscopy in Structural Analysis of Polysaccharides[J].Food and Drug,2007,9(8A):39-43. |
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| Authors: | LIU Yu-hong WANG Feng-shan |
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| Affiliation: | 1. Institute of Biochemical and Biotechnological Drug, School of Pharmaceutical Science, Shandong University, Jinan 250012, China; 2. School of Pharmaceutical Science, Shandong University of Traditional Chinese Medicine, Jinan 250014, China |
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| Abstract: | The structural analysis of polysaccharides is very tedious and difficult because of the structural complexity of the substances,and then the difficulty is a hindrance for the deep study of polysaccharides.Nuclear magnetic resonance(NMR) spectroscopy is the most effective means for the structural analysis of substances and the technology develops at a fast speed in recent years.This article reviews the applications of one-dimensional nuclear magnetic resonance spectroscopy(1D-NMR) and 2D-NMR(COSY,TOCSY,ROESY,NOESY,HMQC,HSQC and HMBCf)or the structural analysis of polysaccharides.The technology can provide information about monosaccharide composition of polysaccharides,residues sequence and its position in glucosidic bond,linkage types,configuration of glycosyl-residues,etc. |
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| Keywords: | nuclear magnetic resonance spectroscopy polysaccharides structural analysis |
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