| 毛细管电泳法分离6种手性化合物 |
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| 引用本文： | 吕迎春,樊竹青,侯云洪.毛细管电泳法分离6种手性化合物[J].云南化工,2014(2):13-16. |
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| 作者姓名： | 吕迎春 樊竹青 侯云洪 |
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| 作者单位： | 普洱学院 生命科学系,云南 普洱665000 |
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| 基金项目： | 云南省教育厅科学研究基金（2013Y108） |
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| 摘 要： | 以羟丙基-β-环糊精作为手性选择剂,采用毛细管电泳法对氧氟沙星、特布他林、克伦特罗、美托洛尔、扁桃酸和苯基丁二酸等6种对映体进行手性分离条件研究。通过对羟丙基-β-环糊精的浓度、缓冲溶液pH值、电压和检测波长等影响手性分离主要因素的探讨,建立了能同时实现对6种对映体手性分离的方法;并采用对照品加入法将手性单体加入到对映体化合物中,确定了氧氟沙星对映体的出峰顺序。
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| 关 键 词： | 毛细管电泳 手性分离 手性化合物 羟丙基-β-环糊精 |
Separation of 6 Chiral Compounds by Capillary Electrophoresis |
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| LV Ying-chun,FAN Zhu-qing,HOU Yun-hong.Separation of 6 Chiral Compounds by Capillary Electrophoresis[J].Yunnan Chemical Technology,2014(2):13-16. |
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| Authors: | LV Ying-chun FAN Zhu-qing HOU Yun-hong |
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| Affiliation: | LV Ying-chun, FAN Zhu-qing, HOU Yun-hong |
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| Abstract: | The enantioseparation conditions of 6 enantiomers including ofloxacin,terbutaline,clen-buterol,metoprolol,aandelic acid and phenyl succinic acid were studied by capillary electrophoresis using HP-β-CD as chiral selector.The effects of the concentration of HP-β-CD,pH of buffer,applied volt-age and detective wave length on chiral separation were investigated.Results showed that some conditions with simple adjustment can separate 6 enantimoers at the same time,therefore the work efficiency was im-proved significantly,and the peaks′sequence of ofloxacin was confirmed by adding chiral monomer into en-antiotomers. |
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| Keywords: | capillary electrophoresis enantioseparation chiral compound hydroxypropyl-β-cy-clodextrin |
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