首页 | 本学科首页   官方微博 | 高级检索  
     

毛细管电泳法分离6种手性化合物
引用本文:吕迎春,樊竹青,侯云洪.毛细管电泳法分离6种手性化合物[J].云南化工,2014(2):13-16.
作者姓名:吕迎春  樊竹青  侯云洪
作者单位:普洱学院 生命科学系,云南 普洱665000
基金项目:云南省教育厅科学研究基金(2013Y108)
摘    要:以羟丙基-β-环糊精作为手性选择剂,采用毛细管电泳法对氧氟沙星、特布他林、克伦特罗、美托洛尔、扁桃酸和苯基丁二酸等6种对映体进行手性分离条件研究。通过对羟丙基-β-环糊精的浓度、缓冲溶液pH值、电压和检测波长等影响手性分离主要因素的探讨,建立了能同时实现对6种对映体手性分离的方法;并采用对照品加入法将手性单体加入到对映体化合物中,确定了氧氟沙星对映体的出峰顺序。

关 键 词:毛细管电泳  手性分离  手性化合物  羟丙基-β-环糊精

Separation of 6 Chiral Compounds by Capillary Electrophoresis
LV Ying-chun,FAN Zhu-qing,HOU Yun-hong.Separation of 6 Chiral Compounds by Capillary Electrophoresis[J].Yunnan Chemical Technology,2014(2):13-16.
Authors:LV Ying-chun  FAN Zhu-qing  HOU Yun-hong
Affiliation:LV Ying-chun, FAN Zhu-qing, HOU Yun-hong
Abstract:The enantioseparation conditions of 6 enantiomers including ofloxacin,terbutaline,clen-buterol,metoprolol,aandelic acid and phenyl succinic acid were studied by capillary electrophoresis using HP-β-CD as chiral selector.The effects of the concentration of HP-β-CD,pH of buffer,applied volt-age and detective wave length on chiral separation were investigated.Results showed that some conditions with simple adjustment can separate 6 enantimoers at the same time,therefore the work efficiency was im-proved significantly,and the peaks′sequence of ofloxacin was confirmed by adding chiral monomer into en-antiotomers.
Keywords:capillary electrophoresis  enantioseparation  chiral compound  hydroxypropyl-β-cy-clodextrin
本文献已被 维普 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号