Characterisation of an antiviral pediocin-like bacteriocin produced by Enterococcus faecium |
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Authors: | Svetoslav Dimitrov Todorov Monica Wachsman Elisabetta Tomé Xavier Dousset Maria Teresa Destro Leon Milner Theodore Dicks Bernadette Dora Gombossy de Melo Franco Manuella Vaz-Velho Djamel Drider |
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Affiliation: | 1. Universidade de São Paulo, Faculdade de Ciências Farmacêuticas, Departamento de Alimentos e Nutrição Experimental, Laboratório de Microbiologia de Alimentos, Av. Prof. Lineu Prestes 580, Bloco 14, 05508-000 São Paulo – SP, Brazil;2. ONIRIS-Ecole Nationale Vétérinaire, Agroalimentaire et de l’Alimentation, Rue de la Géraudière, BP 82225, 44322 Nantes, Cedex 03, France;3. Laboratorio de Virología, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellon 2, Piso 4, C1428EGA Buenos Aires, Argentina;4. Instituto de Ciencia y Tecnología de Alimentos, Escuela de Biología, Universidad Central de Venezuela, Apartado 47.097, Caracas 1041 A, Venezuela;5. Department of Microbiology, University of Stellenbosch, 7600 Stellenbosch, South Africa;6. Escola Superior de Tecnologia e Gestão, Instituto Politécnico de Viana do Castelo, Portugal |
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Abstract: | The bacteriocin-producing strain Enterococcus faecium ST5Ha was isolated from smoked salmon and identified by biomolecular techniques. Ent. faecium ST5Ha produces a pediocin-like bacteriocin with activity against several lactic acid bacteria, Listeria spp. and some other human and food pathogens, and remarkably against HSV-1 virus. Bacteriocin ST5Ha was produced at high levels in MRS broth at 30 °C and 37 °C, reaching a maximum production of 1.0 × 109 AU/ml, checked against Listeria ivanovii ATCC19119 as target strain and surrogate of pathogenic strain Listeria monocytogenes. The molecular weight of bacteriocin ST5Ha was estimated to be 4.5 kDa according to tricine-SDS-PAGE data. Ent. faecium ST5Ha harbors a 1.044 kb chromosomal DNA fragment fitting in size to that of pediocin PA-1/AcH. In addition, the sequencing of bacteriocin ST5Ha gene indicated 99% of DNA homology to pediocin PA-1/AcH. The combined application of low levels (below MIC) of ciprofloxacin and bacteriocin ST5Ha resulted in a synergetic effect in the inhibition of target strain L. ivanovii ATCC19119. Bacteriocin ST5Ha displayed antiviral activity against HSV-1, an important human pathogen, with a selectivity index of 173. To the best of our knowledge, this is the first report on Ent. faecium as a potential producer of pediocin-like bacteriocin with antiviral activity. |
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Keywords: | Pediocin-like bacteriocin Enterococcus faecium Salmon Anti-Listeria activity Antiviral activity |
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