| ns脉冲电场诱导裸小鼠皮下人黑色素移植瘤凋亡 |
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| 引用本文： | 米彦,姚陈果,李成祥,孙才新,唐均英,杨方黎.ns脉冲电场诱导裸小鼠皮下人黑色素移植瘤凋亡[J].高电压技术,2009,35(9):2207-2212. |
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| 作者姓名： | 米彦 姚陈果 李成祥 孙才新 唐均英 杨方黎 |
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| 作者单位： | 1. 重庆大学输配电装备及系统安全与新技术国家重点实验室,重庆,400044
2. 重庆医科大学附属第一医院,重庆,400016 |
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| 基金项目： | 国家自然科学基金重点项目(50637020);;重庆市科委院士专项(CSTC,2007AB3018)~~ |
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| 摘 要： | 为研究ns脉冲电场对在体肿瘤的诱导凋亡效应,以接种人黑色素瘤细胞A375的BALB/c裸小鼠为对象,采用电场强度20kV/cm、脉冲宽度300ns的脉冲电场处理后,抑瘤效应宏观观察到肿瘤组织的生长受到显著抑制(P<0.01),透射电子显微镜观察到典型的凋亡细胞超微结构形态,免疫荧光染色法定性检测到caspase-3蛋白表达明显增强,用RT-PCR方法定量检测到caspase-3 mRNA表达明显增强(P<0.01)。实验结果证实了ns脉冲电场能有效抑制在体肿瘤组织生长,机制在于其通过活化肿瘤细胞caspase-3蛋白酶而诱导肿瘤细胞调亡,为ns脉冲电场治疗肿瘤提供了依据。
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| 关 键 词： | ns脉冲电场 BALB/c裸小鼠 人黑色素瘤细胞A375 凋亡 caspase-3 肿瘤治疗 |
Apoptosis Induction Effects of Nanosecond Pulsed Electric Field on BALB/c Nude Mouse Models Inoculated with Human Melanoma Cells A375 in Vivo |
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| MI Yan
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YAO Chen-guo
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LI Cheng-xiang
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SUN Cai-xin
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TANG Jun-ying
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YANG Fang-li.Apoptosis Induction Effects of Nanosecond Pulsed Electric Field on BALB/c Nude Mouse Models Inoculated with Human Melanoma Cells A375 in Vivo[J].High Voltage Engineering,2009,35(9):2207-2212. |
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| Authors: | MI Yan YAO Chen-guo LI Cheng-xiang SUN Cai-xin TANG Jun-ying YANG Fang-li |
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| Affiliation: | 1. State Key Laboratory of Power Transmission Equipment & System Security and New Technology;Chongqing University;Chongqing 400044;China;2. The First Affiliated Hospital of Chongqing Medical University;Chongqing 400016;China |
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| Abstract: | In order to research the apoptosis induction effects of nanosecond pulsed electric field (nsPEF) in vivo,tumor models in 20 female BALB/c nude mice were established by inoculating with human melanoma cells A375. These mice were randomly divided into treated group (exposed to nsPEF with intensity of 20 kV/cm and duration of 300 ns) and control group equally. 20 days later,compared with that of control group,tumor growth of treated group was effectively (P<0.01) inhibited,typical morphological apoptosis chara... |
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