| 响应面分析法优化肠杆菌的发酵条件生产壳聚糖酶 |
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| 引用本文： | 程仕伟,孙少华,张盈,陶春彦,朱琳,姜国正. 响应面分析法优化肠杆菌的发酵条件生产壳聚糖酶[J]. 四川工业学院学报, 2014, 0(1): 39-42,78 |
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| 作者姓名： | 程仕伟 孙少华 张盈 陶春彦 朱琳 姜国正 |
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| 作者单位： | [1]鲁东大学生命科学学院应用微生物研究所,山东烟台264025 [2]烟台恒源生物股份有限公司,山东烟台265709 |
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| 基金项目： | 山东省高等学校科技计划项目（J12LD02）;鲁东大学学生创新基金（12y039） |
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| 摘 要： | 利用筛选的肠杆菌发酵生产壳聚糖酶，对培养温度、pH和接种量进行优化，确立中心位点后进行响应面设计分析，确定最优发酵培养条件。结果表明：最佳培养工艺条件为培养温度31．74℃、pH6．5和接种量为7．26％（V/V），培养36h时壳聚糖酶活性达到13．59U／mL，实际值与预测值之间的误差约为0．43％。壳聚糖酶的生产方式为延续合成型，具有深入优化并规模生产的潜力。
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| 关 键 词： | 肠杆菌 壳聚糖酶 发酵条件 响应面分析 |
Optimization of Culture Conditions Using the Method of Response Surface Methodology for Chitosanase Production by Enterobacter sp. YT21 |
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| CHENG Shi-wei,SUN Shao-hua,ZHANG Ying,TAO Chun-yan,ZHU Lin,JIANG Guo-zheng. Optimization of Culture Conditions Using the Method of Response Surface Methodology for Chitosanase Production by Enterobacter sp. YT21[J]. Journal of Sichuan University of Science and Technology, 2014, 0(1): 39-42,78 |
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| Authors: | CHENG Shi-wei SUN Shao-hua ZHANG Ying TAO Chun-yan ZHU Lin JIANG Guo-zheng |
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| Affiliation: | 1. Institute of Applied Microbiology, College of Life Sciences, Ludong University, Yantai 264025 China ;2. Yantai tIengyuan Bioengineering Co. Led, gantai 265709 China) |
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| Abstract: | Optimization of culture conditions were conducted for chitosanase production by Enterobacter sp. YT21. Firstly Culture temperature, pH and inoculum volume were optimized by one-factor-at-a-time experiments, and then Box-Behnken design and response surface methodology were employed for further optimization of the chitosanase production. The results indicated that the optimal culture conditions were 31.74℃, pH 6.5 and 7.26% （V/V） inoculum, and the highest chitosanase activity obtained was 13.59 U/mL. Error of the actual value compared with the predicted one was only 0.43% , so Enterobacter sp. YT21 was proved to be a suitable potential microorganism for use in chitosanase production. |
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| Keywords: | Enterobacter sp. chitosanase culture condition response surface methodology |
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