温度诱导双水相分离纯化细菌素工艺条件的选取与优化

利用TritonX-114可萃取疏水性蛋白质的性质，研究了温度诱导双水相分离纯化细菌素乳链菌肽, 用响应面法对成相成份TritonX-114和离子强度进行了优化，最佳浓度分别为3.27% TritonX-114，0.85 mol/L氯化钠. 优化过程中，pH固定为2.0，温度固定为30℃, 在此条件下乳链菌肽的萃取率为92%，纯化系数为9.05.

Optimizing Conditions for Bacteriocin Isolation and Purification in Temperature-induced Aqueous Two-phase Systems

Bacteriocin nisin was isolated and purified in temperature-induced aqueous two-phase systems using the capacity of extracting hydrophobic proteins of TritonX-114. The concentrations of sodium chloride and TritonX-114which can form the aqueous two-phase systems were optimized by the response surface method. The full-factorial design was used to assess the main effects of factors and determine the path of steepest ascent to the optimal region of the aqueous two-phase system composition. The optimum concentrations of TritonX-114 and sodium chloride were then easily determined by using a central composite design and were found to be 3.27% of TritonX-114 and 0.85 mol/L sodium chloride. The recovery and purification factor were 92% and 9.05 respectively under the optimum conditions.