Evaluation of alginate-chitosan semi IPNs as cartilage scaffolds |
| |
Authors: | R Seda T??l? Menem?e Gümü?derelio?lu |
| |
Affiliation: | (1) Chemical Engineering Department, Hacettepe University, Beytepe, 06800 Ankara, Turkey |
| |
Abstract: | In this study, alginate and alginate:chitosan semi interpenetrating polymer network (IPN) scaffolds were prepared by freeze-drying
process. Alginate scaffolds were crosslinked with different concentrations of CaCl2, i.e. 0.5, 1 or 3% (w/v), in 96% (v/v) ethanol solutions for two different periods, i.e. 4 and 24 h, after freeze-drying.
Scanning electron microscope (SEM)/ Energy Dispersive Analysis by X-ray (EDAX) analysis and swelling studies indicated that
crosslinking of scaffolds with 3% (w/v) CaCl2 for 24 h was effectively created suitable alginate scaffolds in terms of optimum porosity and mechanical stability. This
is why, alginate:chitosan semi IPN scaffolds were prepared at the crosslinking condition mentioned above in 70:30, 60:40 and
50:50% (v/v) alginate:chitosan ratios. Besides the attachment and proliferation abilities of ATDC5 murine chondrogenic cells
on alginate, 70:30% (v/v) alginate:chitosan and 50:50% (v/v) alginate:chitosan scaffolds, their cellular responses were assessed
for chondrogenic potential. These structural and cellular outcomes demonstrate potential utility of chitosan semi IPNs in
alginate scaffolds. Comparative results found in relation to alginate scaffolds, support the necessity for alginate:chitosan
scaffolds for improved cartilage tissue engineering. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|