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Feasibility of biohydrogen production from cheese whey using a UASB reactor: Links between microbial community and reactor performance
Authors:E. Castelló  ,C. Garcí  a y Santos,T. Iglesias,G. Paolino,J. Wenzel,L. Borzacconi,C. Etchebehere
Affiliation:1. Chemical Engineering Institute, School of Engineering, University of the Republic, Herrera y Reissig 565, Montevideo, Uruguay;2. Microbiology Department, School of Science and School of Chemistry, University of the Republic, General Flores 2124, Montevideo, Uruguay
Abstract:The present study examines the feasibility of producing hydrogen by dark fermentation using unsterilised cheese whey in a UASB reactor. A lab-scale UASB reactor was operated for more than 250 days and unsterilised whey was used as the feed. The evolution of the microbial community was studied during reactor operation using molecular biology tools (T-RFLP, 16S rRNA cloning library and FISH) and conventional microbiological techniques.
Keywords:Hydrogen production   UASB   Lactose fermentation   Cheese whey
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