The effect of Ni,Fe and Mg concentration on photo-hydrogen production by Rhodopseudomonas faecalis RLD-53 |
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Authors: | Bing-Feng Liu Nan-Qi RenJie Ding Guo-Jun XieWan-Qian Guo |
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Affiliation: | State Key Lab of Urban Water Resource and Environment, Harbin Institute of Technology, School of Municipal and Environmental Engineering, No. 202 Haihe Road, 2nd Campus of HIT Box 2614, Hei Longjiang, Harbin 150090, China |
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Abstract: | In the present study, the effect of Ni2+ (0–10 μmol/l), Fe2+ (0–200 μmol/l) and Mg2+ (0–15 mmol/l) concentration on photo-hydrogen production from acetate was investigated by batch culture. Results showed that under a proper concentration range, Ni2+ was able to enhance the hydrogen production rate and the hydrogen yield; Fe2+ was able to increase the hydrogen yield, and hydrogen production rate was enhanced only when the culturing time was 24–72 h. Ni2+ and Fe2+ at a higher concentration inhibited cell growth. When Ni2+ and Fe2+ concentrations were 4 μmol/l and 80 μmol/l, respectively, maximal hydrogen yield of 2.87 and 2.78 mol H2/mol acetate was obtained when batch culturing at 35 °C with initial pH 7.0. Mg2+ did not significantly affect hydrogen production and hydrogen yield which maintained at about 2.45 mol H2/mol acetate, but it was favorable to cell growth. |
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Keywords: | Ni2+ Fe2+ Mg2+ Photo-hydrogen Rhodopseudomonas faecalis |
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