Enrichment of activated sludge for enhanced hydrogen production from crude glycerol |
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| Authors: | Cristiano Varrone Silvia Rosa Floriana Fiocchetti Barbara Giussani Giulio Izzo Giulia Massini Antonella Marone Antonella Signorini Aijie Wang |
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| Affiliation: | 1. ENEA – Italian Agency for New Technologies, Energy and Sustainable Development, Italy;2. State Key Lab of Urban Water Resource and Environment, Harbin Institute of Technology, China;3. University of Insubria, Dep. of Science and High Technology, Como, Italy |
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| Abstract: | Enriched activated sludge that can effectively convert crude glycerol into bio-hydrogen was selected by an eco-biotechnological approach, in very strict conditions, using biodiesel-derived glycerol as the only carbon source. The thus obtained functional consortium was characterized by the genera Klebsiella, Escherichia/Shigella and Cupriavidus. During enrichment, the dominant metabolic end-product shifted from a 1,3 propanediol to ethanol, with a concomitant increase of the hydrogen yield from 0.18 ± 0.003 to 0.66 ± 0.06 mol/mol and an almost five-fold increase of the hydrogen production. Glycerol degradation efficiency showed an increase of around 50%. In optimized and upscaled conditions it was possible to obtain a hydrogen production rate of 2960 mL H2/L/day ± 185 at a near stoichiometric yield (of 0.90 mol/mol ± 0.01), with a carbon recovery of almost 90%, both in sterile and non-sterile conditions. Glycerol was almost totally degraded (degradation efficiency of 97.42% ± 0.98), independently of the glycerol type used. |
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| Keywords: | Enrichment Crude glycerol Mixed culture Biohydrogen Dark fermentation Biodiesel |
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