营养条件对氧化亚铁硫杆菌生长和脱硫的影响

考察了氧化亚铁硫杆菌（T.f.）对不同氮源、能源的利用,研究了空气中氧化不同时间的煤系黄铁矿的脱硫情况.结果表明：在选择的3种氮源中,尿素经过诱导可被T.f.菌较好地利用,提示该菌可产生诱导性脲酶.在利用尿素初期由于尿素的水解,提高了溶液中的pH值.在选择的能源中,Fe^2＋是T.f.菌容易利用的能源基质.添加培养过的回收单质硫粉可提高菌体生物氧化速率.空气中氧化20 d的煤系黄铁矿有助于菌体的吸附,但吸附菌体的生物氧化速率较低.脱硫过程中30 d后对于空气中氧化4 d的煤系黄铁矿,同时接种亚铁与单质硫培养的菌种其黄铁矿硫脱除率达52.2%,接种亚铁培养的菌种其黄铁矿硫脱除率为43.2%,接种单质硫培养的菌种其黄铁矿硫脱除率为33.9%.

Effect of Nutrients on Growth and Desulphurization with Thiobacillus Ferrooxidans

The utilization of nitrogen and energy sources by Thiobacillus ferrooxidans was investigated, and the removal of sulfur from coal pyrite exposed to the air for different time was studied. The results show that among three kinds of nitrogen sources urea could be used efficiently by Thiobacillus ferrooxidans after induction period, which indicates that the bacterium could produce induced urease. In the systems containing urea pH increased at the earlier stage due to hydrolyzation of urea. The ferrous salts was easy to be used by Thiobacillus ferrooxidans compared with pyrite and element sulfur. The rate of bio-oxidation was significantly enhanced by the reuse of recovered sulfur powder. Pyrite in coal exposed to the air for 20 days was found to enhance the absorption capacity of bacteria with which the bio-oxidation rate was lower. The desulphurization efficiency of pyrite sulfur in coal pyrite exposed for 4 days on the 30th day was 52.2%, 43.2%, 33.9% respectively while simultaneously inoculating the bacteria seeds grown on ferrous iron and element sulfur, single ferrous iron, and single element sulfur respectively.