Impact of Saturated,Polyunsaturated and Monounsaturated Fatty Acid-Rich Micelles on Lipoprotein Synthesis and Secretion in Caco-2 Cells |
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Authors: | Kim G Jackson Paul A Bateman Parveen Yaqoob Christine M Williams |
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Affiliation: | (1) Hugh Sinclair Unit of Human Nutrition, Department of Food and Nutritional Sciences, School of Chemistry, Food and Pharmacy, University of Reading, Reading, RG6 6AP, UK |
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Abstract: | Meal fatty acids have been shown to modulate the size and composition of triacylglycerol (TAG)-rich lipoproteins influencing
the magnitude and duration of the postprandial plasma TAG response. As a result there is considerable interest in the origin
of these meal fatty-acid induced differences in particle composition. Caco-2 cells were incubated over 4 days with fatty acid
mixtures resembling the composition of saturated (SFA), monounsaturated (MUFA) and polyunsaturated fatty acid (PUFA)-rich
meals fed in a previous postprandial study to determine their impact on lipoprotein synthesis and secretion. The MUFA- and
PUFA-rich mixtures supported greater intracellular TAG, but not cholesterol accumulation compared with the SFA-rich mixture
(P < 0.001). The MUFA-rich mixture promoted significantly greater TAG and cholesterol secretion than the other mixtures and
significantly more apolipoprotein B-100 secretion than the PUFA-rich mixture (P < 0.05). Electron microscopy revealed the SFA-rich mixture had led to unfavourable effects on cellular morphology, compared
with the unsaturated fatty acid-rich mixtures. Our findings suggest the MUFA-rich mixture, may support the formation of a
greater number of TAG-rich lipoproteins, which is consistent with indirect observations from our human study. Our electron
micrographs are suggestive that some endocytotic uptake of MUFA-rich taurocholate micelles may promote greater lipoprotein
synthesis and secretion in Caco-2 cells. |
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