Development of a Green Chromatographic Method for Simultaneous Determination of Food Colorants |
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Authors: | Mahnaz Khanavi Mannan Hajimahmoodi Ali Mohammad Ranjbar Mohammad Reza Oveisi Mohammad Reza Shams Ardekani Ghazaleh Mogaddam |
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Affiliation: | (1) Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, P.O. Box: 14155-6451, Tehran, Iran;(2) Drug and Food Control Department, Faculty of Pharmacy, Tehran University of Medical Sciences, P.O. Box: 14155-6451, Tehran, Iran;(3) Department of Traditional Pharmacy, School of Traditional Iranian Medicine, Tehran University of Medical Sciences, Tehran, Iran;(4) Traditional Pharmacy Research Center, Tehran University of Medical Sciences, Tehran, Iran; |
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Abstract: | A green chromatographic method for the successful separation and determination of eight synthetic food colorants (Tartrazine
E 102, Quinoline Yellow E 104, Sunset Yellow E 110, Carmoisine E 122, Ponceau 4R E 124, Allura Red E 129, Indigo Carmine E
132 and Brilliant Blue E 133) was developed. A C8 stationary phase was used and the mobile phase was a mixture of 50 mM phosphate
buffer at pH 7 containing triton X-100 (0.25% v/v). The method was validated as regards its selectivity, linearity, precision, accuracy, limit of detection (LOD) and quantification
(LOQ). LOD of colorants varied between 0.17 μg mL−1 in Allura Red and 1.91 μg mL−1 in Quinoline Yellow. In the case of LOQ, it was ranged from 0.52 in the Allura Red to 5.79 in the Quinoline Yellow. The method
applicability was verified by the determination of colorants present in 22 samples. The 15 samples were only unicolor and
the color concentration in these samples varied from 18.426 ± 0.100 to 610.390 ± 4.711 ppm. The method can be used successfully
to the determination of binary and ternary color food and drug samples too. This method provides substantial green benefits
without using organic solvents in extraction procedure and in both liquid and paper chromatographic methods. |
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