| 改良Genomeshuffling技术选育埃博霉素B高产菌株 |
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| 引用本文： | 龚国利,陈松,李慧,曾桥. 改良Genomeshuffling技术选育埃博霉素B高产菌株[J]. 中国酿造, 2012, 0(11): 42-46 |
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| 作者姓名： | 龚国利 陈松 李慧 曾桥 |
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| 作者单位： | 陕西科技大学生命科学与工程学院;陕西科技大学资源与环境学院 |
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| 基金项目： | 国家自然科学基金(No.20906058);陕西省自然科学基金(No.2010JQ4020);陕西省教育厅自然科学研究专项(No.12JK1023);陕西省科技厅社会发展攻关项目(No.2010K01-200);陕西科技大学学术骨干培育项目(No.XSG2010009) |
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| 摘 要： | 目的：用改genomeshuffling技术选育埃博霉素高产菌株。方法：本研究对genomeshu御ing技术进行了改良，具体是在递归原生质体融合过程中对原生质体进行了紫外诱变；通过UV和DES复合诱变方法获得T4株出发菌株，并用改良genomeshuffling技术选育埃博霉素B高产菌株。结果：通过三轮基因组重组成功选育到了2株遗传稳定的高产埃博霉素B重组菌株，其中一株重组菌株S0073-45的埃博霉素B产量达到T42．5mg／L。结论：该研究证明改良后的基因组重组技术不仅能有效提高埃博霉素B的产量，而且比传统的基因组重组技术更为高效。
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| 关 键 词： | 埃博霉素B 基因组重组 纤维堆囊菌 原生质体诱变 |
Screening and breeding of high epothilone B-producing strains by improvement of genome shuffling |
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| GONG Guoli,CHEN Song,LI Hui,ZENG Qiao. Screening and breeding of high epothilone B-producing strains by improvement of genome shuffling[J]. China Brewing, 2012, 0(11): 42-46 |
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| Authors: | GONG Guoli CHEN Song LI Hui ZENG Qiao |
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| Affiliation: | 1(1.College of Life Science and Engineering,Shanxi University of Science and Technology,Xi’an,710021,China;2.College of Resources and Environment,Shanxi University of Science and Technology,Xi’an,710021,China) |
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| Abstract: | Objective To screen and breed high epothilone B producing strains by improvement of genome shuffling. Methods This study improved the genome shuffling techniques, Protoplast was mutated with UV irradiation in the fusion process; The study using UV and DES compound mutation method screened out four start strains, and high epothilone B-producing strains were obtained by improvement of genome shuffling. Results Two hereditarily stable strains with high epothilone B production were obtained by three cycles of genome shuffling. Among them, strain So 073-45 epothilone B production reached 42.5mg/L. Conclusion The study proves that improvement ofgenome shuffling can not only improve the production of epothilone B, and than traditional genome shuffling more efficient. |
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| Keywords: | epothilone B genome shuffling Sorangium cellulostun protoplast mutagenic |
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