Binding of external ligands onto an engineered virus capsid |
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Authors: | Schmidt, Uli Rudolph, Rainer Bohm, Gerald |
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Affiliation: | 1 Institut für Biotechnologie, Martin-Luther-Universität Halle-Wittenberg, Kurt-Mothes-Str. 3, 06120 Halle and 2 ACGT Progenomics AG, Weinbergweg 22, 06120 Halle, Saale, Germany |
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Abstract: | The development of novel delivery systems for therapeutic substancesincludes targeting of the carriers to a specific site or tissuewithin the body of the recipient. This can be accomplished byappropriate receptor-binding domains and requires linking ofthese domains to the carrier. We have used recombinantly expressedpolyomavirus-like particles as a model system and inserted thesequence of a WW domain into different surface loops of theviral capsid protein VP1. In one variant, the WW domain maintainedits highly selective binding properties of proline-rich ligandsand showed an increased affinity but also an accelerated association/dissociationequilibrium compared to the isolated WW domain, thus allowinga short-term coupling of external ligands onto the surface ofthe virus-like particles. |
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