| 紫杉醇的薄层层析显色反应与应用 |
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| 引用本文： | 范呈浪,王雪松,臧威,李惠,孙剑秋. 紫杉醇的薄层层析显色反应与应用[J]. 中国酿造, 2012, 0(9): 160-162 |
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| 作者姓名： | 范呈浪 王雪松 臧威 李惠 孙剑秋 |
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| 作者单位： | [1]绍兴文理学院生命科学学院,浙江绍兴312000 [2]齐齐哈尔大学生命科学与农林学院,黑龙江齐齐哈尔161006 |
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| 基金项目： | 黑龙江省自然科学基金项目（C200806）;浙江省大学生科技创新活动计划（2011R426008）;绍兴文理学院引进人才科研启动项目（20105021;20105022）;绍兴文理学院学生校级重点科研课题（2012-27） |
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| 摘 要： | 为了进行抗癌药物紫杉醇产生菌株的快速筛选,建立了紫杉醇检测的薄层层析(TLC)方法。研究结果表明,应用薄层层析技术对内生真菌的发酵产物进行检测比高效液相色谱(HPLC)方法更加高效,能够简便、准确的筛选到紫杉醇产生菌株,并且可以初步推测菌株的紫杉醇产生能力。
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| 关 键 词： | 紫杉醇 薄层层析 紫杉醇产生菌株 |
Application of the thin layer chromatography for detection oftaxol |
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| FAN Chenglang,WANG Xuesong,ZANG Wei,LI Hui,SUN Jianqiu. Application of the thin layer chromatography for detection oftaxol[J]. China Brewing, 2012, 0(9): 160-162 |
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| Authors: | FAN Chenglang WANG Xuesong ZANG Wei LI Hui SUN Jianqiu |
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| Affiliation: | 1. Life Science College, Shaoxing University, Shaoxing 312000, China; 2. Life Science College, Qiqihar University, Qiqihaer 161006, China) |
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| Abstract: | In order to screen the taxol-producing fungus rapidly, the thin layer chromatography （TLC） method to detection oftaxol was established. The result showed that the TLC detection was more efficient than high performance liquid chromatography on analysis of fermentation products of fungi. The taxol-producing fungus could be screened simply and accurately by TLC method, and the axol producing ability of fungus could be evalu- ated preliminarily. |
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| Keywords: | taxol thin layer chromatography taxol-producing fungus |
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