| 芽孢杆菌B15胞外蛋白酶和淀粉酶的酶学性质研究 |
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| 引用本文： | 朱薇玲,石豪,李金华,赵子鹏. 芽孢杆菌B15胞外蛋白酶和淀粉酶的酶学性质研究[J]. 中国酿造, 2012, 0(9): 118-121 |
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| 作者姓名： | 朱薇玲 石豪 李金华 赵子鹏 |
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| 作者单位： | [1]武汉工业学院生物与制药工程学院,湖北武汉430023 [2]武汉工业学院省级生物学实验教学示范中心,湖北武汉430023 |
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| 摘 要： | 为探索芽孢杆菌B15胞外蛋白酶和淀粉酶在饲用酶制剂方面的应用,试验对其产酶情况及胞外酶的酶学性质进行了研究。结果显示,该菌株产蛋白酶和淀粉酶的最高酶活性分别为(181.9±3.5)U/mL和(50.2±1.6)U/mL。蛋白酶和淀粉酶的最适作用温度范围分别为40℃～50℃和40℃～70℃,最适作用pH值为7和5～7。蛋白酶和淀粉酶经40℃～50℃热处理,以及在pH值为5～9的缓冲液中较稳定。蛋白酶被EDTA所抑制,属金属蛋白酶,金属离子(10mmol/L)Mg2+和K+对其有激活作用,Fe2+有抑制作用,而Fe2+对淀粉酶有一定的激活作用。
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| 关 键 词： | 芽孢杆菌B15 蛋白酶 淀粉酶 酶学性质 |
Enzymatic properties of extracellular protease and amylase from Bacillus sp. B15 |
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| ZHU Weiling,SHI Hao,LI Jinhua,ZHAO Zipeng. Enzymatic properties of extracellular protease and amylase from Bacillus sp. B15[J]. China Brewing, 2012, 0(9): 118-121 |
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| Authors: | ZHU Weiling SHI Hao LI Jinhua ZHAO Zipeng |
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| Affiliation: | 1.College of Biotechnology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan 430023, China; 2.Hubei Experimental Teaching Demonstration Center of Biology, Wuhan Polytechnic University, Wuhan 430023, China) |
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| Abstract: | Enzymatic activities and properties of extracellular protease and amylase from Bacillus sp. Bt5 were studied for the potential application of the enzymes in feed industry. The results showed that the highest activity ofprotease and amylase were （181.9±3.5）U/ml and （50.2±1.6）U/ml, respectively. The optimum temperature ofprotease and amylase were 40℃-50℃ and 40℃-70℃, respectively. The optimum pH value 7 and 5-7, respectively. They were stable in the ranges 40℃-50℃ and pH value 5-9. The activity of protease was inhibited by metaUoprotease inhibitor （EDTA）, indicating that the protease is a metalloprotease. The protease activity was activated by Mg2＋, K＋ （10mmol/L） and inhibited by Fe2., while the amylase activ- ity was enhanced by Fe2＋. |
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| Keywords: | Bacillus sp. B15 protease amylase enzymatic properties |
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