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酿酒葡萄中内生酵母菌的分离与初步鉴定
引用本文:崔艳莉,蒋雪,祁付云,刘娅. 酿酒葡萄中内生酵母菌的分离与初步鉴定[J]. 中国酿造, 2012, 0(9): 82-84
作者姓名:崔艳莉  蒋雪  祁付云  刘娅
作者单位:[1]新疆农业职业技术学院,新疆昌吉831100 [2]石河子大学食品学院,新疆石河子832000
基金项目:新疆石河子大学(RCZ)(20101)
摘    要:采用组织块培养法,从优质酿酒葡萄品种"赤霞珠"中分离出12株内生酵母菌,其中春季4株,夏季3株,秋季5株;从酿酒葡萄的不同组织部位均分离到了内生酵母菌,其中根部6株、茎部2株、叶部1株、果实3株。经形态、培养特征、子囊孢子和假菌丝的观察及生理生化实验,初步鉴定出这些菌株分别为瓶形酵母和克勒克酵母,其中瓶形酵母6株,克勒克酵母6株。

关 键 词:酿酒葡萄  内生酵母菌  分离  初步鉴定

Isolation and primary identification of endophytic yeast from wine grape
CUI Yanli,JIANG Xue,QI Fuyun,LIU Ya. Isolation and primary identification of endophytic yeast from wine grape[J]. China Brewing, 2012, 0(9): 82-84
Authors:CUI Yanli  JIANG Xue  QI Fuyun  LIU Ya
Affiliation:1.Xinjiang Agricultural Vocational Technical College, Changfi 831100, China; 2.Food College, Shihezi University, Shihezi 832000, China)
Abstract:Twelve endophytic yeasts were isolated sparately from roots, stems and leaves of Xinjiang wine grape by tissue isolation method, in which, four strains were isolated in spring, three strains in summer and five strains in autumn. Among several parts of grape, six strains were seperated from root, two strains from stem, one from leaf and three from fruit. Through morphological appraisal, cultural characteristics, yeast pseudohypha observation, physiological and biochemical determination, these endophytic yeasts were identified as Pityros purom and KIeckera, six strains were Pityros purom, and six were Kleckera.
Keywords:wine grape  endophytic yeast  isolation  primary identification
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