| 生产蜂王酸(10-HDA)菌株的筛选与评价 |
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| 引用本文： | 陶文卿,桂妍雯,王腾飞,王瑞明. 生产蜂王酸(10-HDA)菌株的筛选与评价[J]. 中国酿造, 2012, 31(8): 123-126 |
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| 作者姓名： | 陶文卿 桂妍雯 王腾飞 王瑞明 |
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| 作者单位： | 山东轻工业学院食品与生物工程学院,山东济南,250353 |
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| 摘 要： | 从新鲜的蜂王浆、蜂巢以及其他可能含有脂肪酸的物质中筛选出3株菌株——QYF005、QYF017、QYF024,依据它们产蜂王酸的重现率,确定QYF005作为进一步研究的对象.通过稀释涂布平板的方法,将菌株在30℃培养60h后,选择平板上的单菌落,在160r/min摇床中30℃培养64h,提取产品,用HPLC和GC-MS方法检测该菌株是否在发酵过程中产生蜂王酸.检测结果显示菌株QYF005能够产生蜂王酸,产量达到25mg/L.
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| 关 键 词： | 蜂王酸 菌株筛选 提取 检测 |
Screening and evaluation of microbial strains for the production of 10-HDA |
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| TAO Wenqing , GUI Yanwen , WANG Tengfei , WANG Ruiming. Screening and evaluation of microbial strains for the production of 10-HDA[J]. China Brewing, 2012, 31(8): 123-126 |
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| Authors: | TAO Wenqing GUI Yanwen WANG Tengfei WANG Ruiming |
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| Affiliation: | (Department of Food and Biology Engineenng, Shandong Polytechnic University, Jinan 250353, China) |
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| Abstract: | Three strains-QYF005, QYF017, QYF024 were screened from fresh royal jelly, honeycomb, and other substances possibly containing fatty acids. According to their ability of repeatedly producing 10-HDA, we selected the strain-QYF005 for further study. By dilution and spread plate method, the stains were cultured at 30℃ for 60h. Then the single bacterial colony strain was cultured in shaking table at 30℃, 160r/min for 64h. HPLC and GC-MS methods were used to determine whether they could yield 10-HDA during 64h fermentation in 30℃, 160r/min. The results showed that the strains QYF005 could produce 10-HAD, and the yield reached 25mg/L. |
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| Keywords: | 10-hydroxy-2-decenoic acid（10-HDA） strain screening extraction detection |
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