| H22肝癌细胞与正常小鼠肝细胞胰岛素受体表达的对比 |
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| 引用本文： | 欧晓红,匡安仁,梁正路,王朝俊.H22肝癌细胞与正常小鼠肝细胞胰岛素受体表达的对比[J].同位素,2001,14(2):76-80,86. |
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| 作者姓名： | 欧晓红 匡安仁 梁正路 王朝俊 |
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| 作者单位： | 四川大学华西医院, |
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| 基金项目： | 国家自然科学基金资助项目(39770229) |
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| 摘 要： | 本实验采用氯胺T法进行了胰岛素的
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| 关 键 词： | 胰岛素 受体结构分析 H22肝癌细胞 碘125标记 氯胺T 肝癌 放射治疗药物 基因表达 |
| 文章编号： | 1000-7512(2001)02-0076-05 |
Comparation of the
Expression of Insulin Receptor to H22 Hepatoma Cells With Normal Liver Cells of Mice |
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| OU Xiao-Hong,KUANG An-ren,LIANG Zheng-Lu,WANG Chao-jun.Comparation of the
Expression of Insulin Receptor to H22 Hepatoma Cells With Normal Liver Cells of Mice[J].Isotopes,2001,14(2):76-80,86. |
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| Authors: | OU Xiao-Hong KUANG An-ren LIANG Zheng-Lu WANG Chao-jun |
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| Abstract: | Insulin is labeled with 125I by Ch-T method. Receptor binding assay of 125I-insulin to H22 hepatoma cells and normal mice liver cells are performed respectively. Binding data are calculated according to Scatchard analysis using the ligand program.Statistical comparison is made with the paired t-test. Kd values of H22 hepatoma cells and normal mice liver cells are 1.81±0.56 nmol/L and 2.10±0.91 nmol/L respectively, values of Bmax are 5.61±1.10 amol per cell and 3.22±0.81 amol per cell respectively. H22 hepatoma cells have a significantly higher Bmax than normal mice liver cells(P<0.05), while the two Kd values have little difference(P>0.05). |
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