| 车前子总黄酮的提取工艺优化及体外抗氧化作用研究 |
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| 引用本文： | 万茵,谢明勇,梁丽军,戴和萍. 车前子总黄酮的提取工艺优化及体外抗氧化作用研究[J]. 食品科学, 2006, 27(12): 373-376 |
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| 作者姓名： | 万茵 谢明勇 梁丽军 戴和萍 |
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| 作者单位： | 南昌大学食品科学教育部重点实验室； |
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| 基金项目： | 教育部长江学者和创新团队发展计划（IRT0540）;国家自然科学基金项目（30660226）2004年江西省科技攻关项目 |
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| 摘 要： | 通过正交试验,对溶剂法提取车前子总黄酮化合物的工艺进行了优化研究。实验结果表明,各因素对总黄酮提取率的影响程度由大到小依次为:乙醇浓度>温度>提取次数>固液比。最佳提取工艺条件为:乙醇浓度60%,固液比1:30,提取温度90℃,提取3次,每次2h。用DPPH法和邻苯三酚自氧化法评价了该提取物的体外抗氧化能力。结果表明,车前子总黄酮具有较强的体外抗氧化活性。
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| 关 键 词： | 车前子 总黄酮 提取 抗氧化活性 |
| 文章编号： | 1002-6630（2006）12-0373-04 |
| 收稿时间： | 2006-08-17 |
| 修稿时间： | 2006-08-17 |
Study on the Extraction Technology Optimization and Antioxidative Activity of Flavonoids in Semen Plantaginis |
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| WAN Yin,XIE Ming-yong,LIANG Li-jun,DAI He-ping. Study on the Extraction Technology Optimization and Antioxidative Activity of Flavonoids in Semen Plantaginis[J]. Food Science, 2006, 27(12): 373-376 |
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| Authors: | WAN Yin XIE Ming-yong LIANG Li-jun DAI He-ping |
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| Affiliation: | Key Laboratory of Food Science of Ministry of Education, Nanchang University, Nanchang 330047, China |
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| Abstract: | The solvent extraction technology of flavonoids in Semen Plantaginis was optimized by orthogonal experiments. The experimental results showed that the order of different factors affecting extraction rate was content of ethanol, extraction temperature, extraction time, rate of solid/liquid. The optimal experimental parameters were 60% ethanol content, 90℃ extrac-tion temperature, 1:30 rate of solid/liquid, 2h extraction time, three times. Inv itro antioxidative activity of the extract was evaluated by two systems, viz. DPPH radical scavenging activity and pyrogallol autooxidation methods. The results indicated that flavonoids in Semen Plantaginis have strong antioxidative activity in vitro. |
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| Keywords: | Semen Plantaginis flavonoids extraction antioxidative activity |
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