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Linear phase imaging using differential interference contrast microscopy |
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| Authors: | M. R. Arnison,K. G. Larkin&dagger ,C. J. R. Sheppard,N. I. Smith&Dagger ,& C. J. Cogswell§ |
| Affiliation: | Physical Optics Laboratory, School of Physics, University of Sydney, NSW, 2006, Australia;Canon Information Systems Research Australia, 1 Thomas Holt Drive, North Ryde, NSW, 2113, Australia;Department of Applied Physics, 2-1 Yamadaoka, Suita, Osaka, 565-0871, Japan;Department of Electrical &Computer Engineering, University of Colorado, Boulder, CO 80309-0425, U.S.A. |
| Abstract: | We propose an extension to Nomarski differential interference contrast microscopy that enables isotropic linear phase imaging. The method combines phase shifting, two directions of shear and Fourier‐space integration using a modified spiral phase transform. We simulated the method using a phantom object with spatially varying amplitude and phase. Simulated results show good agreement between the final phase image and the object phase, and demonstrate resistance to imaging noise. |
| Keywords: | Differential interference contrast microscopy Fourier transform Hilbert transform image processing Nomarski differential interference contrast phase retrieval phase shifting spiral phase transform |