| 比光谱-导数分光光度法同时测定对-香豆酸和阿魏酸 |
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| 引用本文： | 赵健,欧仕益.比光谱-导数分光光度法同时测定对-香豆酸和阿魏酸[J].食品科学,2010,31(8):189. |
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| 作者姓名： | 赵健 欧仕益 |
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| 作者单位： | 暨南大学理工学院食品科学与工程系; |
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| 基金项目： | 广东高校科技成果产业化重大项目(cgzhzd0709) |
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| 摘 要： | 根据对-香豆酸和阿魏酸的紫外吸收特点,确立得到两种酚酸稳定的紫外吸收图谱的条件,建立比光谱-导数分光光度法,同时测定对-香豆酸和阿魏酸的含量。在溶剂为乙醇或水或乙醇与水的混合溶液时,调节溶液pH2.0,得到两种酚酸稳定的紫外吸收光谱图,最大吸收峰分别为对-香豆酸308nm、阿魏酸320nm。运用比光谱-导数分光光度法测定对-香豆酸和阿魏酸的二元混合物,回收率在93.40%～103.34%之间,结果良好。对蔗渣碱解提取的酚酸样品进行检测,阿魏酸检测受影响较大,而对-香豆酸检测结果较理想。本法对波谱严重重叠的两种酚酸能进行有效测定,
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| 关 键 词： | 比光谱-导数分光光度法 对-香豆酸 阿魏酸 |
Simultaneous Determination of p-Coumaric Acid and Ferulic Acid Using Ratio Spectra Derivative Spectrophotometry |
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| ZHAO Jian,OU Shi-yi.Simultaneous Determination of p-Coumaric Acid and Ferulic Acid Using Ratio Spectra Derivative Spectrophotometry[J].Food Science,2010,31(8):189. |
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| Authors: | ZHAO Jian OU Shi-yi |
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| Affiliation: | ZHAO Jian,OU Shi-yi(Department of Food Science , Engineering,College of Science , Engineering,Jinan University,Guangzhou 510632,China) |
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| Abstract: | A ratio spectra derivative spectrophotometric method was developed for the simultaneous determination of p-coumaric acid and ferulic acid.p-coumaric acid and ferulic acid dissolved in either ethanol,water or an ethanol aqueous solution with pH adjusted to be 2.0 exhibited stable maximum absorption at 308 nm and 320 nm,respectively.Spike recoveries for both acids were within the range of 93.40%-103.34%.In the determination of p-coumaric acid and ferulic acid by this method in phenolic acids extracted from su... |
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| Keywords: | ratio spectra derivative spectrophotometry p-coumaric acid ferulic acid |
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