| 细菌Sh34的鉴定及其对几种植物病原真菌的拮抗作用测定 |
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| 引用本文： | 董红强,慕卫,刘峰,潘金菊,翟茹环. 细菌Sh34的鉴定及其对几种植物病原真菌的拮抗作用测定[J]. 农药, 2007, 46(11): 779-780 |
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| 作者姓名： | 董红强 慕卫 刘峰 潘金菊 翟茹环 |
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| 作者单位： | 塔里木大学,植物科技学院,新疆,阿拉尔,843300;山东农业大学,植物保护学院,山东,泰安,271018;塔里木大学,植物科技学院,新疆,阿拉尔,843300 |
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| 基金项目： | 山东农业大学青年科技项目 |
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| 摘 要： | 从黄瓜田土壤中分离得到拮抗细菌Sh34,根据其形态特征和生理生化特性及其细菌的16SrDNA序列分析,鉴定其为枯草芽孢杆菌(Bacillus subtilis)。经平皿测试表明,菌株Sh34及其无菌滤液对黄瓜蔓枯病菌(Ascochyta cucumis)、黄瓜枯萎菌(Fusarium oxysporum f.sp.cucumerinum)、黄瓜菌核((Sclerotinia scleotiorum)、瓜果腐霉菌(Pythium aphanidermatum)、立枯丝核菌(Rhixoctonia solani)等多种植物病原真菌均有良好稳定的抑制作用。其无菌滤液能使黄瓜枯萎菌菌丝老化,原生质浓缩。
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| 关 键 词： | 细菌 鉴定 枯草芽孢杆菌 拮抗 |
| 文章编号： | 1006-0413（2007）11-0779-02 |
| 修稿时间： | 2007-02-25 |
Identification of Bacterium Sh34 and Measure of Its Inhibition to Some Pathogenic Fungi |
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| DONG Hong-qiang,MU Wei,LIU Feng,PAN Jin-ju,ZHAI Ru-huan. Identification of Bacterium Sh34 and Measure of Its Inhibition to Some Pathogenic Fungi[J]. Pesticides, 2007, 46(11): 779-780 |
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| Authors: | DONG Hong-qiang MU Wei LIU Feng PAN Jin-ju ZHAI Ru-huan |
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| Affiliation: | l.lnstitute of Plant Science and Technology, Tarim University, Alaer 843300, Xinjiang, China; 2.College of Plant Protection, Shandong Agricultural University, Taian 271018, Shandong, China |
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| Abstract: | The antagonistic bacterial strain Sh34 isolated and screened from field soils of cucumber was identified as Bucillus subtilis according to its morphological biochemical characters and analysis of the partial sequence of the 16SrDNA. Both the bacterial cultural and aseptic filtrate showed activities to inhibit some pathogenic fungi including Ascochyta cucumis, Fusarium oxysporum f.sp.cucumerinum, Sclerotinia scleotiorum, Pythium aphanidermatum, Rhixoctonia solani. on activity plates assay. Its aseptic filtrate is capable to accelerate the hypha to grow older and cytoplasm condensation of Fusarium oxysporum f.sp.. |
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| Keywords: | bacterium identification Bacillus amyloliquefaciens antagonism |
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