响应面法优化葡糖醋杆菌产细菌纤维素的发酵工艺

为提高葡糖醋杆菌生产细菌纤维素的产量,采用Plackett-Burman实验设计和Box-Benhnken Design相结合,对红薯酶解液、起始pH值、装液量等因素进行了研究,优化了产细菌纤维素的发酵工艺.实验结果表明,产细菌纤维素的最佳工艺为:红薯酶解液质量浓度50 g/L、起始pH值6.0、装液量50 mL/(250mL).该工艺条件下得到的细菌纤维素绝干质量(折算成质量浓度)达到4.80g/L,比优化前产量2.20 g/L提高了118％.

Optimization of Gluconacetobacter Fermentation for Bacterial Cellulose Production by Response Surface Method

In order to increase bacterial cellulose production yielded by Gluconacetobacter,the factors including sweet potato hydrolyzate,initial pH,liquid volume in flask and so on by using Plackett-Burman Design and Box-Benhnken Design combination were studied,and the fermentation of bacterial cellulose production was optimized.The results showed that the best conditions for bacterial cellulose production were as follows: sweet potato hydrolyzate of 50 g/L,initial pH 6.0,liquid volume in flask of 50 mL/(250 mL).Under these conditions,the dry weight of the bacterial cellulose reached to 4.80 g/L and increased by 118% compared with 2.20 g/L before optimization.