| 应用电镜原位杂交技术与免疫标记技术研究膀胱上皮细胞的分化 |
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| 引用本文： | 梁凤霞,丁明孝,翟中和.应用电镜原位杂交技术与免疫标记技术研究膀胱上皮细胞的分化[J].电子显微学报,1994(4). |
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| 作者姓名： | 梁凤霞 丁明孝 翟中和 |
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| 作者单位： | 北京大学生命科学学院 |
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| 摘 要： | 本文应用电镜原位杂交技术与免疫标记技术相结合，在超微水平上对ＡＵＭ蛋白及其ｍＲＮＡ在细胞中的分布进行定性与定位的研究。结果显示ＡＵＭ蛋白的基因从小鼠膀胱上皮中间层细胞开始表达，从而为膀胱上皮细胞的分化提供了确凿的证据。
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| 关 键 词： | 膀胱上皮，不对称单位膜，电镜分子原位杂交技术，胶体金免疫标记技术 |
Study on Differentiation of Bladder Urothelium by means of in situ Hybridization and Immuno-labelling Electron Microscopy |
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| Liang Fengxia Ding Mingxiao Zhai Zhonghe.Study on Differentiation of Bladder Urothelium by means of in situ Hybridization and Immuno-labelling Electron Microscopy[J].Journal of Chinese Electron Microscopy Society,1994(4). |
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| Authors: | Liang Fengxia Ding Mingxiao Zhai Zhonghe |
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| Abstract: | The distribution of AUM protein and its mRNA on mouse bladder urothelium has been observed by usingin situ hybridization and immuno-labelling electron microscopy. The results showed that the gene expression ofAUM protein is not only in superficial cells but also in intermidiate cells of bladder epithelium. This presents areliable evidence for the differentiation of bladder urothelium. |
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| Keywords: | asymmetrical unit membrane(AUM)bladder urothelium in situ hybridization for EM immuno-labelling electron microscopy |
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