OPTIMIZATION OF ETHANOL PRODUCTION BY SACCHAROMYCES CEREVISIAE (ATCC 60868) AND PICHIA STIPITIS Y-7124: A RESPONSE SURFACE MODEL FOR SIMULTANEOUS HYDROLYSIS AND FERMENTATION OF WHEAT STRAW |
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| Authors: | V.A. AWAFO D.S. CHAHAL B.K. SIMPSON |
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| Affiliation: | Food Science and Agricultural Chemistry Department McGill University (Macdonald Campus) 21,111 Lakeshore Road Ste Anne de Bellevue, Quebec Canada, H9X3V9;Applied Microbiology Research Center Institut Armand-Frappier Universite du Quebec 531 Boulevard Des Prairies Laval, Quebec Canada, H7N 4Z3 |
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| Abstract: | Optimization models are useful in ascertaining optimum parameters as they relate to a particular response. Ethanol production with different cellulase-systems and substrate concentrations were subjected to this model and the results revealed that crude unextracted cellulase-systems are more efficient than crude extracted cellulase-systems in producing hydrolysates for ethanol production with Pichia stipitis (Y-7124), Saccharomyces cerevisiae (AT 60868) and their mixture. Response surface plots showed that for 80–90% of high concentrations of delignified wheat straw up to 11% could be converted into ethanol by simultaneous hydrolysis with enzyme loading of 16 IU/mL and fermentation with P. stipitis. P. stipitis was found to be the most suitable yeast for the fermentation of mixed hexose and pentose sugars in the wheat straw hydrolysate. |
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