Crystal structure of a trapped phosphoenzyme during a catalytic reaction |
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| Authors: | YH Lee TW Olson CM Ogata DG Levitt LJ Banaszak AJ Lange |
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| Affiliation: | Laboratory of immunogenetics, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, Maryland 20852, USA. |
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| Abstract: | The crystal structure of the fructose-2,6-bisphosphatase domain trapped during the reaction reveal a phosphorylated His 258, and a water molecule immobilized by the product, fructose-6-phosphate. The geometry suggests that the dephosphorylation step requires prior removal of the product for an 'associative in-line' phosphoryl transfer to the catalytic water. |
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