| 毛细管电动色谱法用于DNA水解产物碱基测定 |
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| 引用本文： | 金丽虹,申炳俊,翁占坤,向阳,田坚. 毛细管电动色谱法用于DNA水解产物碱基测定[J]. 长春理工大学学报(自然科学版), 2008, 31(3) |
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| 作者姓名： | 金丽虹 申炳俊 翁占坤 向阳 田坚 |
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| 作者单位： | 长春理工大学,生命科学技术学院,长春,130022;长春理工大学,光电工程学院,长春,130022 |
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| 摘 要： | 采用毛细管电动色谱法对小牛胸腺脱氧核糖核酸(DNA)水解产物中各碱基含量进行定量测量.结果表明:四种碱基的检测限均可达到10-6mol/L数量级,符合生物样品中含量测定的要求.
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| 关 键 词： | 毛细管电动色谱法 碱基 小牛胸腺DNA |
Base Measurement of Hydrolysate of DNA with Capillary Dynamoelectric Chromatography |
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| JIN Lihon,SHEN BingJun,WENG Zhankun,XIANG Yang,TIAN Jian. Base Measurement of Hydrolysate of DNA with Capillary Dynamoelectric Chromatography[J]. Journal of Changchun University of Science and Technology, 2008, 31(3) |
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| Authors: | JIN Lihon SHEN BingJun WENG Zhankun XIANG Yang TIAN Jian |
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| Affiliation: | JIN Lihon,SHEN BingJun,WENG Zhankun,XIANG Yang,TIAN Jian (Changchun University of Science , Technology,Changchun 130022) |
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| Abstract: | Quantitative measurement of four bases contents of hydrolysate of DNA in thymus of infantile ox were tested re-spectively with Capillary Dynamoelectric Chromatography. The results showed that the minimum level of those bases could be as low as 10-6mol/L,which could be met with the requirement for the content in biosamples. |
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| Keywords: | capillary dynamoelectric chromatography base DNA in thymus of infantile ox |
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