A specific,sensitive and high-capacity immunoassay for PAF |
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| Authors: | Brian A Baldo Mary A Smal Alistair C McCaskill |
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| Affiliation: | (1) Kolling Institute of Medical Research, Royal North Shore Hospital of Sydney, St, 2065 Leonards, N.S.W.;(2) Department of Medicine, University of Sydney, 2006 Sydney;(3) Silenus Laboratories, 3122, Victoria, Australia |
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| Abstract: | A specific radioimmunoassay for platelet-activating factor (PAF) sensitive in the range 10–1000 pg (0.02–2 pmoles) has been
developed. Detailed quantitative hapten inhibition studies showed specificity for the acetyl group at C-2 of PAF, a requirement
for the ether linkage at C-1 and some tolerance for substituents on the choline nitrogen. No significant cross-reactivity
was found with phosphatidylcholine and lysophosphatidylcholine or with lysoPAF.
Based on a paper presented at the Third International Conference on Platelet-Activating Factor and Structurally Related Alkyl
Ether Lipids, Tokyo, Japan, May 1989. |
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