顶空气相色谱法快速检测卫生纸中的细菌含量

本文基于细菌在培养过程中的生长繁殖数量与其代谢产物(CO2)释放量的关系,建立了一种利用顶空气相色谱技术快速测定卫生纸中细菌含量的新方法;同时,考察了不同培养基、纸样处理及接种方式、培养液浆浓对检测速度及可靠性的影响。结果表明:采用TGEA培养基比LB液体培养基能获得更快的检测速度;在纸样处理及接种方式中I,SO 8784-1:2005(E)标准的方法优于GB/T 20810-2006标准;为了提高检测的灵敏度和检测的准确性,培养液的纸浆浓度不应高于0.5%(摇床转速360r/min)。在上述条件下,该方法在培养时间8h时即可实现对卫生纸细菌含量的准确检测,与传统方法相比大大提高了检测的效率。

Rapid Determination of Total Bacterial Counts in Hygienic Tissue by Headspace Gas Chromatography

This paper reports a headspace gas chromatographic technique for rapid determination of total bacterial counts in hygienic tissue.The method is based on the relationship between the number of bacteria and the mass of carbon dioxide(CO2) produced during the bacteria incubation.The effects of different conditions such as the types of culture medium,pulp consistency,and approach of sample preparation were investigated.The results showed that compared with Luria Broth(LB) liquid medium,using Tryptone Glucose Extract Agar(TGEA) could accelerate the rate of the bacterial growth and thus the amount of CO2 generated was detectable in a short incubation time.The sample preparation procedures in ISO 8784-1:2005(E) standard method is better than in that of GB/T 20810-2006 standard method since it can transfer much more amount of bacteria into the cultural medium and thus improves the detection sensitivity.A pulp consistency of 0.5% in culture medium was ideal in the present method and the bacterial counts in hygienic tissue can be determined within 8 hours.