Chromatographic fingerprint analysis of Fructus Aurantii Immaturus by HPLC-DAD and chemometric methods |
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| Authors: | XU Xiao-na JIANG Jun-hui LIANG Yi-zeng LI Xiao-ru YI Lun-zhao CHENG Jin-le |
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| Affiliation: | (1) Research Center of Modernization of Traditional Chinese Medicines, College of Chemistry and Chemical Engineering, Central South University, Changsha, 410083, China;(2) Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, Xiangya Hospital, Central South University, Changsha, 410078, China;(3) Diabetes Center, The Second Xiangya Hospital, Central South University, Changsha, 410011, China |
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| Abstract: | An efficient method for quality control of Fructus Aurantii Immaturus (FAI), a famous traditional Chinese medicine (TCM) was established. A simple and reliable high-performance liquid chromatography-photodiode
array detector (HPLC-DAD) procedure coupled with chemometric methods was developed for fingerprint analysis, qualitative analysis
and quantitative determination of this herb. In qualitative and quantitative analyses, heuristic evolving latent projection
(HELP) method was employed to resolve the overlapping peaks of the tested samples. Two bioactive components, namely hesperidin
and naringin, are confirmed and determined, together with four flavonoids compounds tentatively identified including two new
ones. From fingerprint analysis, the fingerprint data were processed with correlation coefficients for quantitative expression
of their similarity and dissimilarity. The developed method based on an integration of chromatographic fingerprint and quantitative
analysis is scientific, and the obtained results can be applied to the quality control of herb medicine. |
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| Keywords: | fingerprint quality control Fructus Aurantii Immaturus high-performance liquid chromatography heuristic evolving latent projection method hesperidin naringin |
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