IgY抗体对幽门螺旋菌菌体抗原细胞毒活性的中和作用

以超声震荡及高速离心法裂解空泡毒素基因阳性 (VacA+ )HP临床分离株 ;采用Hela细胞体外培养法分析此HP菌体蛋白的细胞毒活性及其免疫产卵母鸡制备的IgY抗体的保护作用。HP菌体抗原制备物经SDS PAGE显示至少有十余个蛋白区带 ,主要区带位于约 940 0 0和 6 70 0 0。以此HP菌体抗原包被的ELISA显示 ,球部溃疡患者血清IgG抗体滴度明显增高。体外培养中HP菌体蛋白制备物对Hela细胞具有明显的细胞毒活性 ,LD50 小于 12 μg/ml。以此抗原免疫产卵母鸡 ,提取的IgY抗体浓度依赖地阻断HP菌体蛋白的Hela细胞毒活性 ;提示局部应用抗体制剂做被动免疫 ,有可能成为治疗HP感染性疾病的有效方法

Neutralization Effect of IgY on Cytotoxic Activity of Helicobacterpylory Somatic Antigen

The Vac A + helicobacterpylory(HP) strain isolated from patients was lysated by sonication and ultracentrifugation, and the cytotoxic activity of HP somatic protein, as well as the protective effect of the IgY extracted from the egg yolk laid by the hens immunized with the protein, were detected by the in vitro culture of Hela cells. SDS PAGE proved that HP somatic antigen consisted of at least 10 components, and the molecular weight of main components of it ranged from 67 000 to 94 000. The ELISA using HP somatic antigen as coated antigen proved that the serum IgG titers of the patients with duodenal ulcer were significantly improved. The HP somatic protein showed significant cytotoxic activity to Hela cell, with LD 50 less than 12 μg/ml. The activity could be concentration dependently blocked by the IgY extracted from egg yolk laid by the hens immunized with Hp antigen. It indicated that local administration of IgY might be an effective treatment for HP infectious disease.