Preparation and characterization of high‐molecular‐weight sericin by γ irradiation |
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| Authors: | Nak‐Yun Sung Eui‐Baek Byun Sun‐Kyu Kwon Young‐Choon Yoo Pil‐Mun Jung Jae‐Hun Kim Jong‐Il Choi Mee‐Ree Kim Ju‐Woon Lee |
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| Affiliation: | 1. Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup 580‐185, Republic of Korea;2. Division of Bioresources and Biosciences, Faculty of Agriculture, Graduate School of Kyushu University, 6‐10‐1 Hakozaki, Fukuoka 812‐8581, Japan;3. Department of Microbiology, College of Medicine, Kon‐Yang University, Deajon 302‐718, Republic of Korea;4. Department of Food and Nutrition, Chungnam National University, Daejeon 305‐764, Republic of Korea |
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| Abstract: | We conducted this study to examine the changes in the molecular structure and physiological activities of silk sericin after γ irradiation. Sericin from Bombyx mori was extracted with an Na2CO3 solution. The molecular weight distribution of sericin increased in the gel permeation chromatography and sodium dodecyl sulfate/polyacrylamide gel electrophoresis results as the irradiation dose increased. Circular dichroism data also revealed that the α‐helix contents decreased with the irradiation dose. Ultraviolet absorption was shown a different pattern between the irradiated and unirradiated sericin. However, the Fourier transform infrared spectrum was not changed in all of the groups. Furthermore, the irradiated sericin was significantly increased in 2,2‐diphenyl‐1‐picryl‐hydrazil radical scavenging, and the tyrosinase inhibitory activities increased with irradiation dose. Therefore, γ irradiation was an effective method for producing high‐molecular‐weight sericin and for developing functional foods and cosmetics. © 2010 Wiley Periodicals, Inc. J Appl Polym Sci, 2011 |
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| Keywords: | crosslinking molecular weight distribution/molar mass distribution radiation |
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