Synthesis of a novel histidine‐targeting poly(ethylene glycol) and modification of lysozyme |
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| Authors: | Hong Tian Yuhao Jin Xiangdong Gao Wenbing Yao |
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| Affiliation: | School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, People's Republic of China |
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| Abstract: | A simple and highly efficient method was developed to prepare a synthetic poly(ethylene glycol) (PEG) derivative, which can be used for conjugation to histidine residue. This derivative reacted with sodium nitrite and the intermediate 4‐diazo‐N‐mPEG‐benzamide proved to have a good performance for histidine modification of lysozyme. Then the pegylated lysozyme was separated and its bacteriolytic activities were determined. © 2010 Wiley Periodicals, Inc. J Appl Polym Sci, 2011 |
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| Keywords: | poly(ethylene glycol) functionalization of polymers conjugated polymers histidine separation of polymers |
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