一株纤维素降解新菌种发酵玉米秸秆的生物产氢特性研究

试验从连续流发酵产氢反应器（ZL92114474．1）中分离筛选出一株高效纤维素降解产氢细菌Clostridilan sp．X9。X9利用微晶纤维素（MC）作为发酵产氢底物，得到最大单位体积产氢量（YH2）、比产氢率（YH2／s）和纤维素降解率分别为780mL H2／L-culture、5．1mmol H2／g-cellulose和69．6％。采用酸、碱、氨水和酸化汽爆方式预处理玉米秸秆，结果表明，酸化汽爆方式可以获得最佳的预处理效果。x9利用酸化汽爆玉米秸秆（cSES）发酵产氢的YH2、YH2／s和纤维索降解率分别达到730mL H2／L-culture、4．3mmol H2／g-cellulose和64％。这说明新菌种X9在利用玉米秸秆类生物质纤维素发酵产氢方面具有很好的应用潜力。

SIMULTANEOUS BIO-HYDROGEN PRODUCTION AND PRETREATED CORN STALK DEGRADATION BY A NEW CELLULOSE-DEGRADATION BACTERIUM

In this study,Cellulolyticum sp.X9,anaerobic high efficiency cellulose degrading and hydrogen producing bacterium,was isolated from a continuous-flow H_2 producing reactor(ZL92114474.1).Experimental results showed that strain X9 has high hydrogen yield(Y_(H_2))of 780 ml H_2/L-culture,specific H_2 production rate(Y_(H_2)/s)d 5.1 mmol H_2/ g-cellulose and cellulose degradation degree of 69.6% feeding with microcrystalline cellulose(MC).Then,strain X9 was adopted to investigate H_2 production capabilities dire...