| DNA gel electrophoretic and microaut oradiographic studies on apoptosis in bone tumor cells after exposure with ^153Sm—EDTMP |
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| 引用本文： | ZHuShoupeng. DNA gel electrophoretic and microaut oradiographic studies on apoptosis in bone tumor cells after exposure with ^153Sm—EDTMP[J]. 核技术(英文版), 1999, 10(1): 5-7 |
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| 作者姓名： | ZHuShoupeng |
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| 作者单位： | SuzhouMedicalCollege,Suzhou215007 |
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| 基金项目： | the International Atomic Energy Agency |
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| 摘 要： | The apoptosis in bone tumor cells is studied after ^153Sm-EDTMP irradiation.Fragmented DNA is analyzed by agarose gel electrophoresis.Experimental observations show that 153Sm-EDTMP exposure induces the internucleosomal DNA damage in bone tumor cells the DNA ladder pattern formation in bone tumor cells is show.At the same time,the microautoradiographic study indicates that ^153Sm-EDTMP could permeate through cell membrane and duisplays membrane-seeking condensation in bone tumor cells.Soon afterwards ^153Sm-EDTMP could be phagocytized by the tuymor cells and distributed in cytoplasm as well as nucleus in the form of phagosome.with the prolongation of observing time,the membrane-bounded apoptotic bodies are observed.
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| 关 键 词： | DNA 骨癌细胞 153Sm－EDTMP辐照 |
DNA gel electrophoretic and microaut oradiographic studies on apoptosisin bone tumor cells after exposure with 153Sm-EDTMP |
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| ZHU Shoupeng,XIAO Dong,HAN Xiaofeng. DNA gel electrophoretic and microaut oradiographic studies on apoptosisin bone tumor cells after exposure with 153Sm-EDTMP[J]. , 1999, 10(1): 5-7 |
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| Authors: | ZHU Shoupeng XIAO Dong HAN Xiaofeng |
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| Abstract: | The apoptosis in bone tumor cells is studied after 153Sm-EDTMP irradiation. Fragmented DNA is analyzed by agarose gel electrophoresis. Experimental observations show that 153Sm-EDTMP exposure induces the internucleosomal DNA damage in bone tumor cells the DNA ladder pattern formation in bone tumor cells is shown. At the same time, the microautoradiographic study indicates that 153Sm-EDTMP could permeate through cell membrane and displays membrane-seeking condensation in bone tumor cells. Soon afterwards 153Sm-EDTMP could be phagocytized by the tumor cells and distributed ill cytoplasm as well as nucleus in the form of phagosome. With the prolongation of observing time, the membrane-bounded apoptotic bodies are observed. |
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| Keywords: | DNA gel electrophoresis Microautoradiography Apoptosis Bone tumor cells Irradiation 153Sm-EDTMP |
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